9ijf
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of ATP-dependent diazotase CmaA6== | |
| + | <StructureSection load='9ijf' size='340' side='right'caption='[[9ijf]], [[Resolution|resolution]] 2.73Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9ijf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Kutzneria_albida_DSM_43870 Kutzneria albida DSM 43870]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IJF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ijf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ijf OCA], [https://pdbe.org/9ijf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ijf RCSB], [https://www.ebi.ac.uk/pdbsum/9ijf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ijf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/W5W4E6_9PSEU W5W4E6_9PSEU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although several diazotases have been recently reported, the details of the reaction mechanism are not yet understood. In this study, we investigated the mechanism of CmaA6, an ATP-dependent diazotase, which catalyzes the diazotization of 3-aminocoumaric acid using nitrous acid. X-ray crystallography and cryogenic electron microscopy-single particle analysis revealed CmaA6 structures in the substrate-free and AMP-binding states. Kinetic analysis suggested that CmaA6 catalyzes diazotization via a sequential reaction mechanism in which three substrates (nitrous acid, ATP, and 3-aminocoumaric acid) are simultaneously bound in the reaction pocket. The nitrous acid and 3-aminocoumaric acid binding sites were predicted based on the AMP-binding state and confirmed by site-directed mutagenesis. In addition, computational analysis revealed a tunnel for 3-aminocoumaric acid to enter the reaction pocket, which was advantageous for the sequential reaction mechanism. This study provides important insights into the catalytic mechanism of diazotization in natural product biosynthesis. | ||
| - | + | Structural Basis for the Catalytic Mechanism of ATP-Dependent Diazotase CmaA6.,Kawai S, Karasawa M, Moriwaki Y, Terada T, Katsuyama Y, Ohnishi Y Angew Chem Int Ed Engl. 2025 Apr 24:e202505851. doi: 10.1002/anie.202505851. PMID:40275441<ref>PMID:40275441</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9ijf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Kutzneria albida DSM 43870]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Katsuyama Y]] | ||
| + | [[Category: Kawai S]] | ||
| + | [[Category: Ohnishi Y]] | ||
Current revision
Structure of ATP-dependent diazotase CmaA6
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