1vro

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[[Image:1vro.gif|left|200px]]
[[Image:1vro.gif|left|200px]]
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{{Structure
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|PDB= 1vro |SIZE=350|CAPTION= <scene name='initialview01'>1vro</scene>, resolution 1.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1vro", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=GMS:2&#39;-DEOXYGUANOSINE-5&#39;-MONOSELENOPHOSPHATE'>GMS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene>
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{{STRUCTURE_1vro| PDB=1vro | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vro OCA], [http://www.ebi.ac.uk/pdbsum/1vro PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vro RCSB]</span>
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'''Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure'''
'''Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure'''
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==About this Structure==
==About this Structure==
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1VRO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. This structure supersedes the now removed PDB entry 1N6S. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRO OCA].
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This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1n6s 1n6s]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VRO OCA].
==Reference==
==Reference==
Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475332 12475332]
Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12475332 12475332]
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[[Category: Protein complex]]
 
[[Category: Egli, M.]]
[[Category: Egli, M.]]
[[Category: Pan, C.]]
[[Category: Pan, C.]]
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[[Category: Wawrzak, Z.]]
[[Category: Wawrzak, Z.]]
[[Category: Wilds, C J.]]
[[Category: Wilds, C J.]]
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[[Category: covalent modification of oligonucleotide]]
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[[Category: Covalent modification of oligonucleotide]]
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[[Category: left-handed z-dna]]
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[[Category: Left-handed z-dna]]
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[[Category: multiwavelength anomalous dispersion (mad)]]
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[[Category: Oligonucleotide analogue]]
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[[Category: oligonucleotide analogue]]
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[[Category: Phasing strategy]]
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[[Category: phasing strategy]]
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[[Category: Phosphoroselenoate]]
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[[Category: phosphoroselenoate]]
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[[Category: Synchrotron]]
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[[Category: synchrotron]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:50:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:27:45 2008''
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Revision as of 09:50, 3 May 2008

Image:1vro.gif

Template:STRUCTURE 1vro

Selenium-Assisted Nucleic Acid Crystallography: Use of Phosphoroselenoates for MAD Phasing of a DNA Structure


Overview

The combination of synchrotron radiation and a variety of atoms or ions (either covalently attached to the biomolecule prior to crystallization or soaked into crystals) that serve as anomalous scatterers constitutes a powerful tool in the X-ray crystallographer's repertoire of structure determination techniques. Phosphoroselenoates in which one of the nonbridging phosphate oxygens in the backbone is replaced by selenium offer a simplified means for introducing an anomalous scatterer into oligonucleotides by conventional solid-phase synthesis. Unlike other methods that are used to derivatize DNA or RNA by covalent attachment of a heavy atom (i.e., bromine at the C5 position of pyrimidines), tedious synthesis of specialized nucleosides is not required. Introduction of selenium is readily accomplished in solid-phase oligonucleotide synthesis by replacing the standard oxidation agent with a solution of potassium selenocyanide. This results in a diastereomeric mixture of phosphoroselenoates that can be separated by strong anion-exchange HPLC. As a test case, all 10 DNA hexamers of the sequence CGCGCG containing a single phosphoroselenoate linkage (PSe) were prepared. Crystals were grown for a subset of them, and the structure of [d(C(PSe)GCGCG)](2) was determined by the multiwavelength anomalous dispersion technique and refined to 1.1 A resolution.

About this Structure

This structure supersedes the now removed PDB entry 1n6s. Full crystallographic information is available from OCA.

Reference

Selenium-assisted nucleic acid crystallography: use of phosphoroselenoates for MAD phasing of a DNA structure., Wilds CJ, Pattanayek R, Pan C, Wawrzak Z, Egli M, J Am Chem Soc. 2002 Dec 18;124(50):14910-6. PMID:12475332 Page seeded by OCA on Sat May 3 12:50:27 2008

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