8zaj
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of a 55 kDa nucleoplasmin domain of AtFKBP53== | |
| + | <StructureSection load='8zaj' size='340' side='right'caption='[[8zaj]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8zaj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ZAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ZAJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8zaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8zaj OCA], [https://pdbe.org/8zaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8zaj RCSB], [https://www.ebi.ac.uk/pdbsum/8zaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8zaj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FKB53_ARATH FKB53_ARATH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The knowledge of three-dimensional structures of biological macromolecules is crucial for understanding the molecular mechanisms underlying disease pathology and for devising drugs targeting specific molecules. Single particle cryo-electron microscopy (Cryo-EM) has become indispensable for this purpose, particularly for large macromolecules and their complexes. However, its effectiveness has been limited in achieving near-atomic resolution for smaller macromolecules. This study presents the Cryo-EM structure of a 55 kDa pentameric AtFKBP53 nucleoplasmin domain at 2.0 A nominal resolution. Our approach involves selecting the optimal grid for data collection and precise alignment of small particles to enhance the resolution of the final 3D reconstructed map. In this study, we systematically processed cryo-EM dataset of a small molecule to improve alignment, and this data processing strategy can be used as a guidance to process the cryo-EM data of other small molecules. | ||
| - | + | 2.0 A cryo-EM structure of the 55 kDa nucleoplasmin domain of AtFKBP53.,Bharambe N, Saharan K, Vasudevan D, Basak S J Struct Biol. 2025 Apr 20;217(2):108203. doi: 10.1016/j.jsb.2025.108203. PMID:40262726<ref>PMID:40262726</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8zaj" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Basak S]] | ||
| + | [[Category: Bharambe N]] | ||
| + | [[Category: Saharan K]] | ||
| + | [[Category: Vasudevan D]] | ||
Current revision
Cryo-EM structure of a 55 kDa nucleoplasmin domain of AtFKBP53
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