9iku
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Se-Met CTB10== | |
| + | <StructureSection load='9iku' size='340' side='right'caption='[[9iku]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9iku]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Cercospora_sp._JNU001 Cercospora sp. JNU001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IKU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9iku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9iku OCA], [https://pdbe.org/9iku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9iku RCSB], [https://www.ebi.ac.uk/pdbsum/9iku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9iku ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A977K7H6_9PEZI A0A977K7H6_9PEZI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The creation of enzymes with abiological abilities offers exciting opportunities to access new-to-nature biocatalysis beyond that found in nature. Here, we repurpose a novel protein scaffold, CTB10, as an artificial photoenzyme through genetic code expansion. It enables catalytic deracemization of cyclopropane, a process that remains inaccessible to traditional biocatalysis due to its thermodynamically unfavorable nature. Following structural optimization through directed evolution, a broad substrate scope with high enantioselectivities is achieved. Furthermore, the crystal structure of the CTB10-based photoenzyme-substrate complex well demonstrates how the catalytic chiral cavity is sculpted to promote efficient and selective light-enabled deracemization. Therefore, this study unlocks the potential for achieving challenging deracemization through biocatalysis. | ||
| - | + | Light-Driven Deracemization by a Designed Photoenzyme.,Li M, Zhang Y, Fu K, Deng Z, Yuan Z, Luo Z, Rao Y J Am Chem Soc. 2025 Apr 23;147(16):13190-13199. doi: 10.1021/jacs.4c16521. Epub , 2025 Apr 12. PMID:40219972<ref>PMID:40219972</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Fu | + | <div class="pdbe-citations 9iku" style="background-color:#fffaf0;"></div> |
| - | [[Category: Rao | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cercospora sp. JNU001]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fu K]] | ||
| + | [[Category: Rao YJ]] | ||
Current revision
Crystal structure of Se-Met CTB10
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