9ip0
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of ClpB1 heptamer from Oryza sativa== | |
| + | <StructureSection load='9ip0' size='340' side='right'caption='[[9ip0]], [[Resolution|resolution]] 4.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9ip0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IP0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ip0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ip0 OCA], [https://pdbe.org/9ip0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ip0 RCSB], [https://www.ebi.ac.uk/pdbsum/9ip0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ip0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CLPB1_ORYSJ CLPB1_ORYSJ] Molecular chaperone involved in heat stress response. May play a role in resolubilization of protein aggregates after heat shock.<ref>PMID:12650620</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Heat stress disrupts the protein homeostasis leading to the accumulation of toxic aggregated proteins in the cell. ClpB disaggregase belonging to the AAA+ ATPase superfamily removes the aggregated toxic proteins. ClpB is present ubiquitously in bacteria, yeast, protozoans and plants and plays a role in acquired heat tolerance. This study was focused on cytoplasmic ClpB1 from rice which is the staple food for more than half of world's population. In bacteria and yeast, ClpB forms a hexameric assembly for carrying out the disaggregase function, however, none of the plant ClpB isoforms have been structurally characterized. Here, we report the cryo-EM structure of ClpB1 from rice (Oryza sativa L.; OsClpB1) at 4 A resolution. The structure reveals that OsClpB1 assembles as an unusual heptameric ring, possibly representing a non-processive open conformation. Our results point to the structural plasticity of OsClpB1 since it exists in different oligomeric forms. Analytical ultracentrifugation studies confirmed OsClpB1 exist as a heptamer in solution as well, suggesting the presence of the heptameric form of OsClpB1 within the cellular milieu of the rice plant. | ||
| - | + | Cryo-EM structure provides insights into the unusual heptameric assembly of rice (Oryza sativa L.) ClpB1 AAA+ ATPase.,Jobichen C, Saharan K, Samal A, Choong YK, Jagdev MK, Mohapatra C, Jian S, Babbar R, Dobson RCJ, Grover A, Vasudevan D, Sivaraman J Int J Biol Macromol. 2025 May 3;311(Pt 3):143917. doi: , 10.1016/j.ijbiomac.2025.143917. PMID:40324502<ref>PMID:40324502</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9ip0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oryza sativa Japonica Group]] | ||
| + | [[Category: Jobichen C]] | ||
| + | [[Category: Saharan K]] | ||
| + | [[Category: Sivaraman J]] | ||
| + | [[Category: Vasudevan D]] | ||
Current revision
Cryo-EM structure of ClpB1 heptamer from Oryza sativa
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