9ivw
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Adenovirus serotype 2 100K== | |
| + | <StructureSection load='9ivw' size='340' side='right'caption='[[9ivw]], [[Resolution|resolution]] 3.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9ivw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IVW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.79Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ivw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ivw OCA], [https://pdbe.org/9ivw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ivw RCSB], [https://www.ebi.ac.uk/pdbsum/9ivw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ivw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SHUT_ADE02 SHUT_ADE02] Protein that inhibits host translation while promoting late viral translation by ribosome shunting. Blocks host cap-dependent translation by binding to eIF4G, displacing MKNK1 from cap initiation complexes and preventing EIF4E phosphorylation. Binds to the tripartite leader sequence of viral late mRNAs and recruits host eIF4G, PABPC1/poly-A binding protein and 40S ribosomes subunits on viral mRNAs, allowing ribosome shunting and efficient translation of late viral mRNAs even though conventional translation via ribosome scanning from the cap has been shut off in the host cell. During assembly, acts as a chaperone protein that helps hexon proteins assembly into trimers (Probable).[HAMAP-Rule:MF_04060]<ref>PMID:15220445</ref> <ref>PMID:15314025</ref> <ref>PMID:15827182</ref> <ref>PMID:16081097</ref> <ref>PMID:7159928</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Adenovirus is an icosahedral, non-enveloped DNA virus that infects humans and other animals. The capsid of adenovirus is mainly assembled by the major capsid protein hexon. Folding and assembly of hexon require the viral encoded chaperone 100K, of which the detailed structure and chaperoning mechanism remain unknown. Here, we report the cryoEM structure of 100K in complex with a pre-mature hexon trimer. The structure shows that 100K dimers bind to the bottom double jelly-roll domains of the pre-mature hexon, mainly through a hook-like domain and a loop extruded from the dimerization domain. Additionally, a groove formed at the dimerization interface of 100K accommodates the N-terminal fragment 49-53 of an adjacent hexon protomer. Mutagenesis studies indicate that the interactions at the jelly-roll domain and the N-terminus of hexon are all essential for the proper folding and assembly of hexon. 100K binds and stabilizes the partially folded hexon, preventing premature aggregation of hexon, promoting the folding of the hexon top insertion loops, and facilitating hexon trimerization. | ||
| - | + | Molecular mechanisms of the viral encoded chaperone 100K in capsid folding and assembly of adenovirus.,Li H, Shao L, Liu Z, Liu Q, Xiang Y Nat Commun. 2025 Apr 29;16(1):4013. doi: 10.1038/s41467-025-59301-4. PMID:40301360<ref>PMID:40301360</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9ivw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Human adenovirus 2]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Li H]] | ||
| + | [[Category: Liu Q]] | ||
| + | [[Category: Xiang Y]] | ||
Current revision
Structure of Adenovirus serotype 2 100K
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