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| | <SX load='6nn3' size='340' side='right' viewer='molstar' caption='[[6nn3]], [[Resolution|resolution]] 3.22Å' scene=''> | | <SX load='6nn3' size='340' side='right' viewer='molstar' caption='[[6nn3]], [[Resolution|resolution]] 3.22Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nn3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/B19_virus B19 virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=6mf7 6mf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NN3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NN3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nn3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_parvovirus_B19 Human parvovirus B19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NN3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NN3 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10798 B19 virus])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.22Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nn3 OCA], [http://pdbe.org/6nn3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nn3 RCSB], [http://www.ebi.ac.uk/pdbsum/6nn3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nn3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nn3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nn3 OCA], [https://pdbe.org/6nn3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nn3 RCSB], [https://www.ebi.ac.uk/pdbsum/6nn3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nn3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q75U93_PAVHB Q75U93_PAVHB] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 19: |
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| | ==See Also== | | ==See Also== |
| | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] |
| | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: B19 virus]] | |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| | + | [[Category: Human parvovirus B19]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Klose, T]] | + | [[Category: Klose T]] |
| - | [[Category: Liu, Y]] | + | [[Category: Liu Y]] |
| - | [[Category: Rossmann, M G]] | + | [[Category: Rossmann MG]] |
| - | [[Category: Sun, Y]] | + | [[Category: Sun Y]] |
| - | [[Category: Antibody]]
| + | |
| - | [[Category: B19]]
| + | |
| - | [[Category: Epitope]]
| + | |
| - | [[Category: Virus like particle]]
| + | |
| - | [[Category: Virus like particle-immune system complex]]
| + | |
| - | [[Category: Vp2]]
| + | |
| Structural highlights
Function
Q75U93_PAVHB
Publication Abstract from PubMed
Parvovirus B19, one of the most common human pathogens, is a small DNA virus that belongs to the Parvoviridae As a result of previous infections, antibodies to B19 are present in most adults. B19 has a strong tropism to erythroid progenitor cells and is able to cause a series of medical conditions including fifth disease, arthritis, myocarditis, hydrops fetalis and aplastic crisis. No approved vaccine is currently available for B19 and there is a lack of structural characterization of any B19 epitopes. Here we present the first cryo-EM structure of a B19 virus-like particle (VLP) complexed with the antigen-binding fragment (Fab) of a human neutralizing antibody, 860-55D. A model was built into the 3.2A resolution map and the antigenic residues on the surface of B19 capsid were identified. Antibody 860-55D bridges the capsid of B19 by binding to a quaternary structure epitope formed by residues from three neighboring VP2 capsid proteins.IMPORTANCE Parvovirus B19 is a common human pathogen and a particular threat to children, pregnant women, and patients with sickle cell disease or AIDS. Currently neutralizing antibody is the most efficient treatment of acute B19 infections. Research on the antigenic properties of B19 will guide the usage of these antibodies and facilitate vaccine development. We have determined and report here the structure of B19 VLPs complexed with the Fab of a human neutralizing antibody at high resolution. The structure shows a quaternary structure epitope formed by three VP2 proteins and provides details on host recognition of human B19 virus.
Structure of parvovirus B19 decorated by Fabs from a human antibody.,Sun Y, Klose T, Liu Y, Modrow S, Rossmann MG J Virol. 2019 Feb 20. pii: JVI.01732-18. doi: 10.1128/JVI.01732-18. PMID:30787153[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Sun Y, Klose T, Liu Y, Modrow S, Rossmann MG. Structure of parvovirus B19 decorated by Fabs from a human antibody. J Virol. 2019 Feb 20. pii: JVI.01732-18. doi: 10.1128/JVI.01732-18. PMID:30787153 doi:http://dx.doi.org/10.1128/JVI.01732-18
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