1w18
From Proteopedia
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'''CRYSTAL STRUCTURE OF LEVANSUCRASE FROM GLUCONACETOBACTER DIAZOTROPHICUS''' | '''CRYSTAL STRUCTURE OF LEVANSUCRASE FROM GLUCONACETOBACTER DIAZOTROPHICUS''' | ||
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[[Category: Tarbouriech, N.]] | [[Category: Tarbouriech, N.]] | ||
[[Category: Taylor, E J.]] | [[Category: Taylor, E J.]] | ||
| - | [[Category: | + | [[Category: Fructosyl transferase]] |
| - | [[Category: | + | [[Category: Glycosyltransferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:00:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:00, 3 May 2008
CRYSTAL STRUCTURE OF LEVANSUCRASE FROM GLUCONACETOBACTER DIAZOTROPHICUS
Overview
The endophytic Gram-negative bacterium Gluconacetobacter diazotrophicus SRT4 secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10), which converts sucrose into fructooligosaccharides and levan. The enzyme is included in GH (glycoside hydrolase) family 68 of the sequence-based classification of glycosidases. The three-dimensional structure of LsdA has been determined by X-ray crystallography at a resolution of 2.5 A (1 A=0.1 nm). The structure was solved by molecular replacement using the homologous Bacillus subtilis (Bs) levansucrase (Protein Data Bank accession code 1OYG) as a search model. LsdA displays a five-bladed beta-propeller architecture, where the catalytic residues that are responsible for sucrose hydrolysis are perfectly superimposable with the equivalent residues of the Bs homologue. The comparison of both structures, the mutagenesis data and the analysis of GH68 family multiple sequences alignment show a strong conservation of the sucrose hydrolytic machinery among levansucrases and also a structural equivalence of the Bs levansucrase Ca2+-binding site to the LsdA Cys339-Cys395 disulphide bridge, suggesting similar fold-stabilizing roles. Despite the strong conservation of the sucrose-recognition site observed in LsdA, Bs levansucrase and GH32 family Thermotoga maritima invertase, structural differences appear around residues involved in the transfructosylation reaction.
About this Structure
1W18 is a Single protein structure of sequence from Gluconacetobacter diazotrophicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus., Martinez-Fleites C, Ortiz-Lombardia M, Pons T, Tarbouriech N, Taylor EJ, Arrieta JG, Hernandez L, Davies GJ, Biochem J. 2005 Aug 15;390(Pt 1):19-27. PMID:15869470 Page seeded by OCA on Sat May 3 13:00:49 2008
