1w36

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[[Image:1w36.gif|left|200px]]
[[Image:1w36.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1w36 |SIZE=350|CAPTION= <scene name='initialview01'>1w36</scene>, resolution 3.1&Aring;
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The line below this paragraph, containing "STRUCTURE_1w36", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+E'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1w36| PDB=1w36 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w36 OCA], [http://www.ebi.ac.uk/pdbsum/1w36 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w36 RCSB]</span>
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}}
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'''RECBCD:DNA COMPLEX'''
'''RECBCD:DNA COMPLEX'''
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[[Category: Singleton, M R.]]
[[Category: Singleton, M R.]]
[[Category: Wigley, D B.]]
[[Category: Wigley, D B.]]
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[[Category: dna repair]]
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[[Category: Dna repair]]
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[[Category: helicase]]
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[[Category: Helicase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: nuclease]]
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[[Category: Nuclease]]
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[[Category: recombination]]
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[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:05:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:30:30 2008''
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Revision as of 10:05, 3 May 2008

Template:STRUCTURE 1w36

RECBCD:DNA COMPLEX


Overview

RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. RecBCD is a bipolar helicase that splits the duplex into its component strands and digests them until encountering a recombinational hotspot (Chi site). The nuclease activity is then attenuated and RecBCD loads RecA onto the 3' tail of the DNA. Here we present the crystal structure of RecBCD bound to a DNA substrate. In this initiation complex, the DNA duplex has been split across the RecC subunit to create a fork with the separated strands each heading towards different helicase motor subunits. The strands pass along tunnels within the complex, both emerging adjacent to the nuclease domain of RecB. Passage of the 3' tail through one of these tunnels provides a mechanism for the recognition of a Chi sequence by RecC within the context of double-stranded DNA. Gating of this tunnel suggests how nuclease activity might be regulated.

About this Structure

1W36 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks., Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB, Nature. 2004 Nov 11;432(7014):187-93. PMID:15538360 Page seeded by OCA on Sat May 3 13:05:41 2008

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