1w4b

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[[Image:1w4b.jpg|left|200px]]
[[Image:1w4b.jpg|left|200px]]
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{{Structure
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|PDB= 1w4b |SIZE=350|CAPTION= <scene name='initialview01'>1w4b</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1w4b", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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{{STRUCTURE_1w4b| PDB=1w4b | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w4b OCA], [http://www.ebi.ac.uk/pdbsum/1w4b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w4b RCSB]</span>
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}}
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'''P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)'''
'''P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)'''
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[[Category: Stuart, D I.]]
[[Category: Stuart, D I.]]
[[Category: Tuma, R.]]
[[Category: Tuma, R.]]
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[[Category: hexameric helicase]]
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[[Category: Hexameric helicase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: molecular motor]]
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[[Category: Molecular motor]]
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[[Category: non-hydrolysable atp analogue]]
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[[Category: Non-hydrolysable atp analogue]]
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[[Category: packaging atpase]]
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[[Category: Packaging atpase]]
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[[Category: virus dsrna]]
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[[Category: Virus dsrna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:08:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:30:59 2008''
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Revision as of 10:08, 3 May 2008

Image:1w4b.jpg

Template:STRUCTURE 1w4b

P4 PROTEIN FROM PHI12 IN COMPLEX WITH PRODUCT (AMPCPP MG 22C)


Overview

Many viruses package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi12, located at the vertices of the icosahedral capsid, is such a packaging motor. We have captured crystallographic structures of P4 for all the key points along the catalytic pathway, including apo, substrate analog bound, and product bound. Substrate and product binding have been observed as both binary complexes and ternary complexes with divalent cations. These structures reveal large movements of the putative RNA binding loop, which are coupled with nucleotide binding and hydrolysis, indicating how ATP hydrolysis drives RNA translocation through cooperative conformational changes. Two distinct conformations of bound nucleotide triphosphate suggest how hydrolysis is activated by RNA binding. This provides a model for chemomechanical coupling for a prototype of the large family of hexameric helicases and oligonucleotide translocating enzymes.

About this Structure

1W4B is a Single protein structure of sequence from Pseudomonas phage phi12. Full crystallographic information is available from OCA.

Reference

Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation., Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI, Cell. 2004 Sep 17;118(6):743-55. PMID:15369673 Page seeded by OCA on Sat May 3 13:08:16 2008

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