5h0r
From Proteopedia
(Difference between revisions)
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A0S1LIW6_CPVBM A0A0S1LIW6_CPVBM] | [https://www.uniprot.org/uniprot/A0A0S1LIW6_CPVBM A0A0S1LIW6_CPVBM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed. | ||
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| + | Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5h0r" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</SX> | </SX> | ||
Current revision
RNA dependent RNA polymerase ,vp4,dsRNA
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Categories: Bombyx mori cypovirus 1 | Cypovirus | Dendrolimus punctatus cypovirus 1 | Large Structures | Chen W | Cheng L | Li X | Liu H | Wang J | Wang X | Xu B | Zhou N | Zhu B
