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| | <SX load='6hyp' size='340' side='right' viewer='molstar' caption='[[6hyp]], [[Resolution|resolution]] 4.40Å' scene=''> | | <SX load='6hyp' size='340' side='right' viewer='molstar' caption='[[6hyp]], [[Resolution|resolution]] 4.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6hyp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HYP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HYP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MDN1, REA1, YLR106C, L2901, L8004.13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hyp OCA], [https://pdbe.org/6hyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hyp RCSB], [https://www.ebi.ac.uk/pdbsum/6hyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hyp ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hyp OCA], [http://pdbe.org/6hyp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hyp RCSB], [http://www.ebi.ac.uk/pdbsum/6hyp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hyp ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/MDN1_YEAST MDN1_YEAST] Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. Mediates ATP-dependent remodeling of the pre-ribosome just prior to export to allow maturation of 60S subunits into export-competent particles. Removes the ribosome biogenesis factor RSA4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. Involved in 3' processing of the 5.8S rRNA.<ref>PMID:15260980</ref> <ref>PMID:15528184</ref> <ref>PMID:19737519</ref> <ref>PMID:20542003</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boland, A]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Busselez, J]] | + | [[Category: Boland A]] |
| - | [[Category: Fagiewicz, R]] | + | [[Category: Busselez J]] |
| - | [[Category: Papai, G]] | + | [[Category: Fagiewicz R]] |
| - | [[Category: Schmidt, H]] | + | [[Category: Papai G]] |
| - | [[Category: Sosnowski, P]] | + | [[Category: Schmidt H]] |
| - | [[Category: Urnavicius, L]] | + | [[Category: Sosnowski P]] |
| - | [[Category: Aaa+ protein]]
| + | [[Category: Urnavicius L]] |
| - | [[Category: Mdn1]]
| + | |
| - | [[Category: Midasin]]
| + | |
| - | [[Category: Molecular machine]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Rea1]]
| + | |
| - | [[Category: Ribosome maturation]]
| + | |
| Structural highlights
Function
MDN1_YEAST Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. Mediates ATP-dependent remodeling of the pre-ribosome just prior to export to allow maturation of 60S subunits into export-competent particles. Removes the ribosome biogenesis factor RSA4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. Involved in 3' processing of the 5.8S rRNA.[1] [2] [3] [4]
Publication Abstract from PubMed
The biogenesis of 60S ribosomal subunits is initiated in the nucleus where rRNAs and proteins form pre-60S particles. These pre-60S particles mature by transiently interacting with various assembly factors. The ~5000 amino-acid AAA+ ATPase Rea1 (or Midasin) generates force to mechanically remove assembly factors from pre-60S particles, which promotes their export to the cytosol. Here we present three Rea1 cryoEM structures. We visualise the Rea1 engine, a hexameric ring of AAA+ domains, and identify an alpha-helical bundle of AAA2 as a major ATPase activity regulator. The alpha-helical bundle interferes with nucleotide-induced conformational changes that create a docking site for the substrate binding MIDAS domain on the AAA +ring. Furthermore, we reveal the architecture of the Rea1 linker, which is involved in force generation and extends from the AAA+ ring. The data presented here provide insights into the mechanism of one of the most complex ribosome maturation factors.
The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.,Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H Elife. 2018 Nov 26;7. pii: 39163. doi: 10.7554/eLife.39163. PMID:30460895[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nissan TA, Galani K, Maco B, Tollervey D, Aebi U, Hurt E. A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol Cell. 2004 Jul 23;15(2):295-301. PMID:15260980 doi:http://dx.doi.org/10.1016/j.molcel.2004.06.033
- ↑ Galani K, Nissan TA, Petfalski E, Tollervey D, Hurt E. Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem. 2004 Dec 31;279(53):55411-8. Epub 2004 Nov 3. PMID:15528184 doi:http://dx.doi.org/10.1074/jbc.M406876200
- ↑ Ulbrich C, Diepholz M, Bassler J, Kressler D, Pertschy B, Galani K, Bottcher B, Hurt E. Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits. Cell. 2009 Sep 4;138(5):911-22. PMID:19737519 doi:http://dx.doi.org/S0092-8674(09)00792-2
- ↑ Bassler J, Kallas M, Pertschy B, Ulbrich C, Thoms M, Hurt E. The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly. Mol Cell. 2010 Jun 11;38(5):712-21. doi: 10.1016/j.molcel.2010.05.024. PMID:20542003 doi:http://dx.doi.org/10.1016/j.molcel.2010.05.024
- ↑ Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H. The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1. Elife. 2018 Nov 26;7. pii: 39163. doi: 10.7554/eLife.39163. PMID:30460895 doi:http://dx.doi.org/10.7554/eLife.39163
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