9lmo

From Proteopedia

(Difference between revisions)

Current revision

Cryo-EM structure of human apo inactive GPR4

Structural highlights

9lmo is a 4 chain structure with sequence from Escherichia coli, Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GPR4_HUMAN Proton-sensing G-protein coupled receptor couples to multiple intracellular signaling pathways, including GNAS/cAMP, GNAQ/phospholipase C (PLC), and GNA12/GNA13/Rho pathways (PubMed:12955148, PubMed:17462861, PubMed:20211729, PubMed:22110680). Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells through the GNA12/GNA13/Rho GTPase signaling pathway (PubMed:32058960). In the brain may mediate central respiratory sensitivity to CO(2)H(+) (By similarity).[UniProtKB:Q8BUD0]^[1] ^[2] ^[3] ^[4] ^[5] C562_ECOLX Electron-transport protein of unknown function.

References

↑ Ludwig MG, Vanek M, Guerini D, Gasser JA, Jones CE, Junker U, Hofstetter H, Wolf RM, Seuwen K. Proton-sensing G-protein-coupled receptors. Nature. 2003 Sep 4;425(6953):93-8. PMID:12955148 doi:10.1038/nature01905
↑ Tobo M, Tomura H, Mogi C, Wang JQ, Liu JP, Komachi M, Damirin A, Kimura T, Murata N, Kurose H, Sato K, Okajima F. Previously postulated "ligand-independent" signaling of GPR4 is mediated through proton-sensing mechanisms. Cell Signal. 2007 Aug;19(8):1745-53. PMID:17462861 doi:10.1016/j.cellsig.2007.03.009
↑ Liu JP, Nakakura T, Tomura H, Tobo M, Mogi C, Wang JQ, He XD, Takano M, Damirin A, Komachi M, Sato K, Okajima F. Each one of certain histidine residues in G-protein-coupled receptor GPR4 is critical for extracellular proton-induced stimulation of multiple G-protein-signaling pathways. Pharmacol Res. 2010 Jun;61(6):499-505. PMID:20211729 doi:10.1016/j.phrs.2010.02.013
↑ Chen A, Dong L, Leffler NR, Asch AS, Witte ON, Yang LV. Activation of GPR4 by acidosis increases endothelial cell adhesion through the cAMP/Epac pathway. PLoS One. 2011;6(11):e27586. PMID:22110680 doi:10.1371/journal.pone.0027586
↑ Krewson EA, Sanderlin EJ, Marie MA, Akhtar SN, Velcicky J, Loetscher P, Yang LV. The Proton-Sensing GPR4 Receptor Regulates Paracellular Gap Formation and Permeability of Vascular Endothelial Cells. iScience. 2020 Feb 21;23(2):100848. PMID:32058960 doi:10.1016/j.isci.2020.100848

1 Structural highlights
2 Function
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/9lmo"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9lmo is ON HOLD  until Paper Publication
+==Cryo-EM structure of human apo inactive GPR4==
+<StructureSection load='9lmo' size='340' side='right'caption='[[9lmo]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9lmo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LMO FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lmo OCA], [https://pdbe.org/9lmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lmo RCSB], [https://www.ebi.ac.uk/pdbsum/9lmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lmo ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GPR4_HUMAN GPR4_HUMAN] Proton-sensing G-protein coupled receptor couples to multiple intracellular signaling pathways, including GNAS/cAMP, GNAQ/phospholipase C (PLC), and GNA12/GNA13/Rho pathways (PubMed:12955148, PubMed:17462861, PubMed:20211729, PubMed:22110680). Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells through the GNA12/GNA13/Rho GTPase signaling pathway (PubMed:32058960). In the brain may mediate central respiratory sensitivity to CO(2)H(+) (By similarity).[UniProtKB:Q8BUD0]<ref>PMID:12955148</ref> <ref>PMID:17462861</ref> <ref>PMID:20211729</ref> <ref>PMID:22110680</ref> <ref>PMID:32058960</ref> [https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli]]
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Chen LN]]
+[[Category: Xi K]]
+[[Category: Zhou H]]

9lmo

From Proteopedia

Current revision

Cryo-EM structure of human apo inactive GPR4

Structural highlights

Function

References

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