1w85

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[[Image:1w85.gif|left|200px]]
[[Image:1w85.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1w85 |SIZE=350|CAPTION= <scene name='initialview01'>1w85</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1w85", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Tdp+Binding+Site+For+Chain+G'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TDP:THIAMIN+DIPHOSPHATE'>TDP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_dehydrogenase_(acetyl-transferring) Pyruvate dehydrogenase (acetyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.1 1.2.4.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1w85| PDB=1w85 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w85 OCA], [http://www.ebi.ac.uk/pdbsum/1w85 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w85 RCSB]</span>
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}}
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'''THE CRYSTAL STRUCTURE OF PYRUVATE DEHYDROGENASE E1 BOUND TO THE PERIPHERAL SUBUNIT BINDING DOMAIN OF E2'''
'''THE CRYSTAL STRUCTURE OF PYRUVATE DEHYDROGENASE E1 BOUND TO THE PERIPHERAL SUBUNIT BINDING DOMAIN OF E2'''
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Pyruvate dehydrogenase (acetyl-transferring)]]
 
[[Category: Frank, R A.W.]]
[[Category: Frank, R A.W.]]
[[Category: Luisi, B F.]]
[[Category: Luisi, B F.]]
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[[Category: Perham, R N.]]
[[Category: Perham, R N.]]
[[Category: Pratap, J V.]]
[[Category: Pratap, J V.]]
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[[Category: acetyl transferase]]
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[[Category: Acetyl transferase]]
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[[Category: dehydrogenase]]
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[[Category: Dehydrogenase]]
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[[Category: dihydrolipoyl]]
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[[Category: Dihydrolipoyl]]
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[[Category: multienzyme complex]]
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[[Category: Multienzyme complex]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: pyruvate]]
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[[Category: Pyruvate]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:17:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:32:32 2008''
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Revision as of 10:17, 3 May 2008

Image:1w85.gif

Template:STRUCTURE 1w85

THE CRYSTAL STRUCTURE OF PYRUVATE DEHYDROGENASE E1 BOUND TO THE PERIPHERAL SUBUNIT BINDING DOMAIN OF E2


Overview

Thiamine diphosphate (ThDP) is used as a cofactor in many key metabolic enzymes. We present evidence that the ThDPs in the two active sites of the E1 (EC 1.2.4.1) component of the pyruvate dehydrogenase complex communicate over a distance of 20 angstroms by reversibly shuttling a proton through an acidic tunnel in the protein. This "proton wire" permits the co-factors to serve reciprocally as general acid/base in catalysis and to switch the conformation of crucial active-site peptide loops. This synchronizes the progression of chemical events and can account for the oligomeric organization, conformational asymmetry, and "ping-pong" kinetic properties of E1 and other thiamine-dependent enzymes.

About this Structure

1W85 is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

A molecular switch and proton wire synchronize the active sites in thiamine enzymes., Frank RA, Titman CM, Pratap JV, Luisi BF, Perham RN, Science. 2004 Oct 29;306(5697):872-6. PMID:15514159 Page seeded by OCA on Sat May 3 13:17:26 2008

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