9hdb
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Sla1 SH3_3 domain (residues 355-414)== | |
| + | <StructureSection load='9hdb' size='340' side='right'caption='[[9hdb]], [[Resolution|resolution]] 1.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9hdb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HDB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.491Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hdb OCA], [https://pdbe.org/9hdb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hdb RCSB], [https://www.ebi.ac.uk/pdbsum/9hdb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hdb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SLA1_YEAST SLA1_YEAST] Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.<ref>PMID:8335689</ref> <ref>PMID:8756649</ref> <ref>PMID:9008707</ref> <ref>PMID:9128251</ref> <ref>PMID:10198057</ref> <ref>PMID:10594004</ref> <ref>PMID:11940605</ref> <ref>PMID:11950888</ref> <ref>PMID:12388763</ref> <ref>PMID:12237851</ref> <ref>PMID:12814545</ref> <ref>PMID:12734398</ref> <ref>PMID:14761940</ref> <ref>PMID:15525671</ref> <ref>PMID:17286805</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The interaction network of Sla2, a vital endocytic mid-coat adaptor protein, undergoes constant rearrangement. Sla2 serves as a scaffold linking the membrane to the actin cytoskeleton, with its role modulated by the clathrin light chain (CLC), which inhibits Sla2's function under certain conditions. We show that Sla2 has two independent binding sites for CLC: one previously described in homologs of fungi (Sla2) and metazoa (Hip1R), and a second found only in Fungi. We present the structural model of the Sla2 actin-binding domains in the context of regulatory structural domains by cryoelectron microscopy. We provide an interaction map of Sla2 and the regulatory proteins Sla1 and Pan1, predicted by AI modeling and confirmed by molecular biophysics techniques. Pan1 may compete with CLC for the conserved Sla2-binding site. These results enhance the mapping of crucial interactions at endocytic checkpoints and highlight the divergence between Metazoa and Fungi in this vital process. | ||
| - | + | Sla2 is a core interaction hub for clathrin light chain and the Pan1/End3/Sla1 complex.,Draper-Barr G, Defelipe LA, Ruiz-Carrillo D, Gustavsson E, Landau M, Garcia-Alai M Structure. 2025 Apr 28:S0969-2126(25)00147-9. doi: 10.1016/j.str.2025.04.013. PMID:40347949<ref>PMID:40347949</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9hdb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Defelipe LA]] | ||
| + | [[Category: Draper-Barr G]] | ||
| + | [[Category: Garcia-Alai MM]] | ||
| + | [[Category: Ruiz-Carillo D]] | ||
Current revision
Sla1 SH3_3 domain (residues 355-414)
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