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1zlm

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(New page: 200px<br /> <applet load="1zlm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zlm, resolution 1.07&Aring;" /> '''Crystal structure o...)
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Revision as of 18:29, 12 November 2007

Image:1zlm.gif

1zlm, resolution 1.07Å

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Crystal structure of the SH3 domain of human osteoclast stimulating factor

Overview

Osteoclast-stimulating factor (OSF) is an intracellular signaling protein, produced by osteoclasts themselves, that enhances osteoclast formation and, bone resorption. It is thought to act via an Src-related signaling pathway, and contains SH3 and ankyrin-repeat domains which are involved in, protein-protein interactions. As part of a structure-based anti-bone-loss, drug-design program, the atomic resolution X-ray structure of the, recombinant human OSF SH3 domain (hOSF-SH3) has been determined. The, domain, residues 12-72, yielded crystals that diffracted to the ultrahigh, resolution of 1.07 A. The overall structure shows a characteristic SH3, fold consisting of two perpendicular beta-sheets that form a beta-barrel., Structure-based sequence alignment reveals that the putative proline-rich, peptide-binding site of hOSF-SH3 consists of (i) residues that are highly, conserved in the SH3-domain family, including residues Tyr21, Phe23, Trp49, Pro62, Asn64 and Tyr65, and (ii) residues that are less conserved, and/or even specific to hOSF, including Thr22, Arg26, Thr27, Glu30, Asp46, Thr47, Asn48 and Leu60, which might be key to designing specific, inhibitors for hOSF to fight osteoporosis and related bone-loss diseases., There are a total of 13 well defined water molecules forming hydrogen, bonds with the above residues in and around the peptide-binding pocket., Some of those water molecules might be important for drug-design, approaches. The hOSF-SH3 structure at atomic resolution provides an, accurate framework for structure-based design of its inhibitors.

About this Structure

1ZLM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the SH3 domain of human osteoclast-stimulating factor at atomic resolution., Chen L, Wang Y, Wells D, Toh D, Harold H, Zhou J, DiGiammarino E, Meehan EJ, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):844-8. Epub 2006 Aug 18. PMID:16946461

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