1wbf
From Proteopedia
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'''WINGED BEAN LECTIN, SACCHARIDE FREE FORM''' | '''WINGED BEAN LECTIN, SACCHARIDE FREE FORM''' | ||
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[[Category: Srinivas, V R.]] | [[Category: Srinivas, V R.]] | ||
[[Category: Suguna, K.]] | [[Category: Suguna, K.]] | ||
| - | [[Category: | + | [[Category: Blood group specificity]] |
| - | [[Category: lectin | + | [[Category: Legume lectin]] |
| - | [[Category: | + | [[Category: Protein crystallography]] |
| - | + | [[Category: Saccharide free form]] | |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:25:13 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 10:25, 3 May 2008
WINGED BEAN LECTIN, SACCHARIDE FREE FORM
Overview
The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop.
About this Structure
1WBF is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.
Reference
Structure of basic winged-bean lectin and a comparison with its saccharide-bound form., Manoj N, Srinivas VR, Suguna K, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310 Page seeded by OCA on Sat May 3 13:25:13 2008
