1wcq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1wcq.gif|left|200px]]
[[Image:1wcq.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1wcq |SIZE=350|CAPTION= <scene name='initialview01'>1wcq</scene>, resolution 2.10&Aring;
+
The line below this paragraph, containing "STRUCTURE_1wcq", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=AC1:Dan+Binding+Site+For+Chain+C'>AC1</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=DAN:2-DEOXY-2,3-DEHYDRO-N-ACETYL-NEURAMINIC+ACID'>DAN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1wcq| PDB=1wcq | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wcq OCA], [http://www.ebi.ac.uk/pdbsum/1wcq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wcq RCSB]</span>
+
-
}}
+
'''MUTAGENESIS OF THE NUCLEOPHILIC TYROSINE IN A BACTERIAL SIALIDASE TO PHENYLALANINE.'''
'''MUTAGENESIS OF THE NUCLEOPHILIC TYROSINE IN A BACTERIAL SIALIDASE TO PHENYLALANINE.'''
Line 30: Line 27:
[[Category: Taylor, G.]]
[[Category: Taylor, G.]]
[[Category: Watson, J N.]]
[[Category: Watson, J N.]]
-
[[Category: glycosidase]]
+
[[Category: Glycosidase]]
-
[[Category: hydrolase neuraminidase]]
+
[[Category: Hydrolase neuraminidase]]
-
[[Category: micromonospora viridifacien]]
+
[[Category: Micromonospora viridifacien]]
-
[[Category: sialidase]]
+
[[Category: Sialidase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:28:33 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:27 2008''
+

Revision as of 10:28, 3 May 2008

Image:1wcq.gif

Template:STRUCTURE 1wcq

MUTAGENESIS OF THE NUCLEOPHILIC TYROSINE IN A BACTERIAL SIALIDASE TO PHENYLALANINE.


Overview

Mutants of the Micromonospora viridifaciens sialidase, Y370E and Y370F, are catalytically active retaining enzymes that operate by different mechanisms. Previous substitutions with smaller amino acids, including Y370D, yielded inverting sialidases. At least one water molecule can fit into the active-site cavity of this mutant and act as a nucleophile from the face opposite the leaving group (Biochemistry 2003, 42, 12 682). Thus, addition of a CH(2) unit (Asp versus Glu) changes the mechanism from inversion back to retention of configuration. Based on Bronsted beta(lg) values, it is proposed that the Y370E mutant reacts by a double-displacement mechanism (beta(lg) on k(cat)/K(m) -0.36+/-0.04) with Glu370 acting as the nucleophile. However, the Y370F mutant (beta(lg) on k(cat)/K(m) -0.79+/-0.12) reacts via a dissociative transition state. The crystal structure of the Y370F mutant complexed with 2-deoxy-2,3-dehydro-N-acetylneuraminic acid shows no significant active-site perturbation relative to the wild-type enzyme.

About this Structure

1WCQ is a Single protein structure of sequence from Micromonospora viridifaciens. Full crystallographic information is available from OCA.

Reference

Two nucleophilic mutants of the Micromonospora viridifaciens sialidase operate with retention of configuration by two different mechanisms., Watson JN, Newstead S, Narine AA, Taylor G, Bennet AJ, Chembiochem. 2005 Nov;6(11):1999-2004. PMID:16206228 Page seeded by OCA on Sat May 3 13:28:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wcq"
Personal tools