1wcr

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[[Image:1wcr.gif|left|200px]]
[[Image:1wcr.gif|left|200px]]
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{{Structure
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|PDB= 1wcr |SIZE=350|CAPTION= <scene name='initialview01'>1wcr</scene>
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The line below this paragraph, containing "STRUCTURE_1wcr", creates the "Structure Box" on the page.
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span>
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{{STRUCTURE_1wcr| PDB=1wcr | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wcr OCA], [http://www.ebi.ac.uk/pdbsum/1wcr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wcr RCSB]</span>
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'''TRIMERIC STRUCTURE OF THE ENZYME IIA FROM ESCHERICHIA COLI PHOSPHOTRANSFERASE SYSTEM SPECIFIC FOR N,N'-DIACETYLCHITOBIOSE'''
'''TRIMERIC STRUCTURE OF THE ENZYME IIA FROM ESCHERICHIA COLI PHOSPHOTRANSFERASE SYSTEM SPECIFIC FOR N,N'-DIACETYLCHITOBIOSE'''
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Solution structure of enzyme IIA(Chitobiose) from the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system., Tang C, Williams DC Jr, Ghirlando R, Clore GM, J Biol Chem. 2005 Mar 25;280(12):11770-80. Epub 2005 Jan 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15654077 15654077]
Solution structure of enzyme IIA(Chitobiose) from the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system., Tang C, Williams DC Jr, Ghirlando R, Clore GM, J Biol Chem. 2005 Mar 25;280(12):11770-80. Epub 2005 Jan 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15654077 15654077]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Clore, G M.]]
[[Category: Clore, G M.]]
[[Category: Tang, C.]]
[[Category: Tang, C.]]
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[[Category: iia]]
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[[Category: Iia]]
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[[Category: mutagenesis,chitobiose]]
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[[Category: Mutagenesis,chitobiose]]
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[[Category: phosphotransferase]]
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[[Category: Phosphotransferase]]
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[[Category: pt]]
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[[Category: Pt]]
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[[Category: sugar transport]]
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[[Category: Sugar transport]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:28:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:29 2008''
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Revision as of 10:28, 3 May 2008

Image:1wcr.gif

Template:STRUCTURE 1wcr

TRIMERIC STRUCTURE OF THE ENZYME IIA FROM ESCHERICHIA COLI PHOSPHOTRANSFERASE SYSTEM SPECIFIC FOR N,N'-DIACETYLCHITOBIOSE


Overview

The solution structure of trimeric Escherichia coli enzyme IIA(Chb) (34 kDa), a component of the N,N'-diacetylchitobiose/lactose branch of the phosphotransferase signal transduction system, has been determined by NMR spectroscopy. Backbone residual dipolar couplings were used to provide long range orientational restraints, and long range (|i - j| > or = 5 residues) nuclear Overhauser enhancement restraints were derived exclusively from samples in which at least one subunit was 15N/13C/2H/(Val-Leu-Ile)-methyl-protonated. Each subunit consists of a three-helix bundle. Hydrophobic residues lining helix 3 of each subunit are largely responsible for the formation of a parallel coiled-coil trimer. The active site histidines (His-89 from each subunit) are located in three symmetrically placed deep crevices located at the interface of two adjacent subunits (A and C, C and B, and B and A). Partially shielded from bulk solvent, structural modeling suggests that phosphorylated His-89 is stabilized by electrostatic interactions with the side chains of His-93 from the same subunit and Gln-91 from the adjacent subunit. Comparison with the x-ray structure of Lactobacillus lactis IIA(Lac) reveals some substantial structural differences, particularly in regard to helix 3, which exhibits a 40 degrees kink in IIA(Lac) versus a 7 degrees bend in IIA(Chb). This is associated with the presence of an unusually large (230-angstroms3) buried hydrophobic cavity at the trimer interface in IIA(Lac) that is reduced to only 45 angstroms3) in IIA(Chb).

About this Structure

1WCR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Solution structure of enzyme IIA(Chitobiose) from the N,N'-diacetylchitobiose branch of the Escherichia coli phosphotransferase system., Tang C, Williams DC Jr, Ghirlando R, Clore GM, J Biol Chem. 2005 Mar 25;280(12):11770-80. Epub 2005 Jan 14. PMID:15654077 Page seeded by OCA on Sat May 3 13:28:36 2008

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