Journal:IUCrJ:S2052252525006645

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Laser parameters for photoactivation are systematically explored, and time-resolved structures over timescales ranging from 100 µs to 1.4 s using synchrotron and XFEL beamlines are described. The effective use of this photocage for time-resolved crystallography is demonstrated with laser powers as low as 0.19 µJ (~0.1 nJ∙µm^-2), resulting in high occupancy NO-bound protein structures. Appropriate illumination conditions for such experiments are determined, and considerations for more general reaction initiation using photocages are discussed. An example is seen in the
Laser parameters for photoactivation are systematically explored, and time-resolved structures over timescales ranging from 100 µs to 1.4 s using synchrotron and XFEL beamlines are described. The effective use of this photocage for time-resolved crystallography is demonstrated with laser powers as low as 0.19 µJ (~0.1 nJ∙µm^-2), resulting in high occupancy NO-bound protein structures. Appropriate illumination conditions for such experiments are determined, and considerations for more general reaction initiation using photocages are discussed. An example is seen in the
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high laser intensity illumination (1600 mJ, PDB: [[9htc]]) McCP-β maps and chain A, <scene name='10/1087716/020_Fig_S8a/1'>2Fo minus Fc</scene> and <scene name='10/1087716/020_Fig_S8b/1'>omit</scene>, contoured at 1σ and 5σ respectively. In this experiment, the fixed target chip was reversed in orientation compared to the other structures in this manuscript, and Mylar film was used to seal the chip, with the result that far less light reached the sample.
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Results of a high laser intensity illumination (1600 mJ, PDB: [[9htc]]) McCP-β showing maps and chain A, for a <scene name='10/1087716/020_Fig_S8a/1'>2Fo minus Fc</scene> and for an <scene name='10/1087716/020_Fig_S8b/1'>omit</scene>, contoured at 1σ and 5σ respectively. In this experiment, the fixed target chip was reversed in orientation compared to the other structures in this manuscript, and Mylar film was used to seal the chip, with the result that far less light reached the sample.
<b>References</b><br>
<b>References</b><br>

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