1zsd

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Revision as of 18:33, 12 November 2007

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spinqualitylabelsall models 1zsd, resolution 1.700Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure Of HLA-B*3501 Presenting an 11-Mer EBV Antigen EPLPQGQLTAY

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

MHC class I molecules generally present peptides of 8-10 aa long, forming, an extended coil in the HLA cleft. Although longer peptides can also bind, to class I molecules, they tend to bulge from the cleft and it is not, known whether the TCR repertoire has sufficient plasticity to recognize, these determinants during the antiviral CTL response. In this study, we, show that unrelated individuals infected with EBV generate a significant, CTL response directed toward an HLA-B*3501-restricted, 11-mer epitope from, the BZLF1 Ag. The 11-mer determinant adopts a highly bulged conformation, with seven of the peptide side chains being solvent-exposed and available, for TCR interaction. Such a complex potentially creates a structural, challenge for TCR corecognition of both HLA-B*3501 and the peptide Ag., Surprisingly, unrelated B*3501 donors recognizing the 11-mer use identical, or closely related alphabeta TCR sequences that share particular CDR3, motifs. Within the small number of dominant CTL clonotypes observed, each, has discrete fine specificity for the exposed side chain residues of the, peptide. The data show that bulged viral peptides are indeed immunogenic, but suggest that the highly constrained TCR repertoire reflects a limit to, TCR diversity when responding to some unusual MHC peptide ligands.

Disease

Known diseases associated with this structure: Abacavir hypersensitivity, susceptibility to OMIM:[142830], Hypoproteinemia, hypercatabolic OMIM:[109700], Spondyloarthropathy, susceptibility to, 1 OMIM:[142830], Stevens-Johnson syndrome, carbamazepine-induced, susceptibility to OMIM:[142830]

About this Structure

1ZSD is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

CTL recognition of a bulged viral peptide involves biased TCR selection., Miles JJ, Elhassen D, Borg NA, Silins SL, Tynan FE, Burrows JM, Purcell AW, Kjer-Nielsen L, Rossjohn J, Burrows SR, McCluskey J, J Immunol. 2005 Sep 15;175(6):3826-34. PMID:16148129

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