1wky

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[[Image:1wky.gif|left|200px]]
[[Image:1wky.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1wky |SIZE=350|CAPTION= <scene name='initialview01'>1wky</scene>, resolution 1.65&Aring;
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The line below this paragraph, containing "STRUCTURE_1wky", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1wky| PDB=1wky | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wky OCA], [http://www.ebi.ac.uk/pdbsum/1wky PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wky RCSB]</span>
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}}
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'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''
'''Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module'''
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==About this Structure==
==About this Structure==
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1WKY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA].
==Reference==
==Reference==
Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272186 15272186]
Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272186 15272186]
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[[Category: Bacillus sp.]]
 
[[Category: Mannan endo-1,4-beta-mannosidase]]
[[Category: Mannan endo-1,4-beta-mannosidase]]
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[[Category: Protein complex]]
 
[[Category: Akita, M.]]
[[Category: Akita, M.]]
[[Category: Grant, W D.]]
[[Category: Grant, W D.]]
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[[Category: Takeda, N.]]
[[Category: Takeda, N.]]
[[Category: Yamamoto, M.]]
[[Category: Yamamoto, M.]]
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[[Category: catalytic domain]]
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[[Category: Catalytic domain]]
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[[Category: cbm]]
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[[Category: Cbm]]
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[[Category: tim barrel]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:49:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:37:37 2008''
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Revision as of 10:49, 3 May 2008

Image:1wky.gif

Template:STRUCTURE 1wky

Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module


Overview

An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:15272186 Page seeded by OCA on Sat May 3 13:49:17 2008

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