9jwn
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of FrCas9 in complex with sgRNA and 43-bp dsDNA substrate== | |
| - | + | <StructureSection load='9jwn' size='340' side='right'caption='[[9jwn]], [[Resolution|resolution]] 2.89Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[9jwn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Faecalibaculum_rodentium Faecalibaculum rodentium] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JWN FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.89Å</td></tr> | |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jwn OCA], [https://pdbe.org/9jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jwn RCSB], [https://www.ebi.ac.uk/pdbsum/9jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jwn ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| - | [[Category: | + | == Function == |
| - | [[Category: Chen | + | [https://www.uniprot.org/uniprot/A0A1Q9YNK3_9FIRM A0A1Q9YNK3_9FIRM] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480] |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Faecalibaculum rodentium]] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen SD]] | ||
| + | [[Category: Liu SQ]] | ||
| + | [[Category: Yang M]] | ||
Current revision
Cryo-EM structure of FrCas9 in complex with sgRNA and 43-bp dsDNA substrate
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