1zv6
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(New page: 200px<br /> <applet load="1zv6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zv6" /> '''NMR structure of the human dematin headpiec...)
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Revision as of 18:35, 12 November 2007
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NMR structure of the human dematin headpiece S74E mutant
Overview
Dematin is an actin binding protein from the junctional complex of the, erythrocyte cytoskeleton. The protein has two actin binding sites and, bundles actin filaments in vitro. This actin bundling activity is, reversibly regulated by phosphorylation in the carboxyl terminal, "headpiece" domain (DHP). DHP is a typical villin-type headpiece actin, binding motif and contains a flexible N-terminal loop and an alpha-helical, C-terminal subdomain that is phosphorylated at Ser74. The NMR structure of, a Ser74-to-Glu mutant (DHPs74e) closely mimics the conformation of, phosphorylated DHP. The negative charge at Ser74 does not alter the, conformation of the C-terminal subdomain, but attracts the N-terminal loop, toward the C terminus, changing the orientation of the N-terminal, subdomain. NMR relaxation studies also indicate reduced mobility in the, N-terminal loop in DHPs74e. Thus, phosphorylation in DHP serves as a, switch controlling the conformational state of DHP and the actin bundling, activity of dematin.
About this Structure
1ZV6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A phosphorylation-induced conformation change in dematin headpiece., Jiang ZG, McKnight CJ, Structure. 2006 Feb;14(2):379-87. PMID:16472756
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