1wq2
From Proteopedia
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'''Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)''' | '''Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)''' | ||
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[[Category: Tanaka, I.]] | [[Category: Tanaka, I.]] | ||
[[Category: Voordouw, G.]] | [[Category: Voordouw, G.]] | ||
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Revision as of 10:59, 3 May 2008
Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)
Overview
Dissimilatory sulfite reductase D (DsrD) from Desulfovibrio vulgaris has been crystallized for a neutron diffraction study. The initial crystals obtained were too small for the neutron experiment. In order to obtain a larger crystal (>1 mm3), a combination of two techniques was developed to determine the optimum crystallization conditions: a crystallization phase diagram was obtained, followed by crystal-quality assessment via X-ray diffraction. Using conditions determined in this manner, a large single crystal (1.7 mm3) of DsrD protein was subsequently grown in D(2)O solution by the macroseeding technique. A neutron diffraction experiment was carried out using the BIX-3 diffractometer at the Japan Atomic Energy Research Institute (JAERI), collecting data to 2.4 A resolution from an optimized crystal.
About this Structure
1WQ2 is a Single protein structure of sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris., Chatake T, Mizuno N, Voordouw G, Higuchi Y, Arai S, Tanaka I, Niimura N, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2306-9. Epub 2003, Nov 27. PMID:14646103 Page seeded by OCA on Sat May 3 13:59:55 2008
