8yy2
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Kinesin-14 in nucleotide-free state bound to 13 PF Microtubule== | |
| + | <StructureSection load='8yy2' size='340' side='right'caption='[[8yy2]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8yy2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8YY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8YY2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8yy2 OCA], [https://pdbe.org/8yy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8yy2 RCSB], [https://www.ebi.ac.uk/pdbsum/8yy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8yy2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Kinesin motor proteins hydrolyze ATP to produce force for spindle assembly and vesicle transport, performing essential functions in cell division and motility, but the structural changes required for force generation are uncertain. We now report high-resolution structures showing new transitions in the kinesin mechanochemical cycle, including power stroke fluctuations upon ATP binding and a post-hydrolysis state with bound ADP + free phosphate. We find that rate-limiting ADP release occurs upon microtubule binding, accompanied by central beta-sheet twisting, which triggers the power stroke - stalk rotation and neck mimic docking - upon ATP binding. Microtubule release occurs with beta-strand-to-loop transitions, implying that beta-strand refolding induces Pi release and the recovery stroke. The strained beta-sheet during the power stroke and strand-to-loop transitions identify the beta-sheet as the long-sought motor spring. | ||
| - | + | Structural transitions in kinesin minus-end directed microtubule motility.,Shibata S, Wang MY, Imasaki T, Shigematsu H, Wei Y, Jobichen C, Hagio H, Sivaraman J, Endow SA, Nitta R bioRxiv [Preprint]. 2024 Jul 29:2024.07.29.605428. doi: , 10.1101/2024.07.29.605428. PMID:39131399<ref>PMID:39131399</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8yy2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Drosophila melanogaster]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sus scrofa]] | ||
| + | [[Category: Endow SA]] | ||
| + | [[Category: Imasaki T]] | ||
| + | [[Category: Nitta R]] | ||
| + | [[Category: Shibata S]] | ||
| + | [[Category: Shigematsu H]] | ||
Current revision
Kinesin-14 in nucleotide-free state bound to 13 PF Microtubule
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