9w3i
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of Oryza sativa phosphate transporter SPDT in apo-state== | |
| + | <StructureSection load='9w3i' size='340' side='right'caption='[[9w3i]], [[Resolution|resolution]] 3.16Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9w3i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Indica_Group Oryza sativa Indica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9W3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9W3I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.16Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9w3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9w3i OCA], [https://pdbe.org/9w3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9w3i RCSB], [https://www.ebi.ac.uk/pdbsum/9w3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9w3i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A1Y0BRS7_ORYSI A0A1Y0BRS7_ORYSI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Phosphorus (P) limitation severely affects crop yields. To address the molecular basis of inorganic phosphate (Pi) specific transport within the sulfate transporter (SULTR) family, we determined the cryo-electron microscopy structures of Oryza sativa SULTR-like phosphorus distribution transporter (OsSPDT), a key Pi transporter for grain allocation, in apo- and Pi-binding states. OsSPDT forms a domain-swapped homodimer with each protomer containing an N-terminal domain (NTD), a transmembrane domain (TMD) divided into core and gate subdomains, and a C-terminal sulfate transporter and antisigma factor (STAS) domain. The structure adopts a cytoplasm-facing conformation with Pi coordinated at the core-gate interface. Key residues, including SPDT-unique Ser(170), mediate Pi specificity within the binding pocket, distinguishing it evolutionarily from sulfate transporters within the SULTR family. Domain-swapping and mutational studies demonstrate functional interdependence of the NTD, TMD, and STAS domains. This work elucidates Pi selectivity in plant SULTR transporters and provides a molecular basis for developing low-phytate rice via OsSPDT gene editing. | ||
| - | + | Molecular mechanism underlying phosphate distribution by SULTR family transporter SPDT in Oryza sativa.,Fang S, Zhao Y, Zhang X, Yu F, Zhang P Sci Adv. 2025 Oct 10;11(41):eady3442. doi: 10.1126/sciadv.ady3442. Epub 2025 Oct , 10. PMID:41071878<ref>PMID:41071878</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9w3i" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oryza sativa Indica Group]] | ||
| + | [[Category: Fang S]] | ||
| + | [[Category: Zhang P]] | ||
| + | [[Category: Zhang X]] | ||
Current revision
Cryo-EM structure of Oryza sativa phosphate transporter SPDT in apo-state
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