9kov
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of an amidohydrolase mutant from Thermonema== | |
| + | <StructureSection load='9kov' size='340' side='right'caption='[[9kov]], [[Resolution|resolution]] 2.56Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9kov]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrinomonas_methylaliphatogenes Pyrinomonas methylaliphatogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KOV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kov OCA], [https://pdbe.org/9kov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kov RCSB], [https://www.ebi.ac.uk/pdbsum/9kov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kov ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Ochratoxin A (OTA) is highly toxic and widely distributed, posing serious threats to human and animal health. Searching for effective OTA-detoxifying enzyme is crucial for the prevention and control of OTA contaminations. Here, a new OTA-detoxifying enzyme, TrADH from Thermonema rossianum is identified, which exhibits highest temperature tolerance among OTA-detoxifying enzymes. TrADH maintains good activity in the range of 45-85 degrees C and retains about 50 % activity after heating at 70 degrees C for 30 min. Based on the solved crystal structures, the catalytic mechanism is proposed, and protein engineering of catalytic-related residues is performed to obtain a 2.1-fold upgraded variant TrADHS67E with the specific enzyme activity of 3990 U/mg, which is more efficient than the reported OTA-detoxifying enzymes. The efficient degradation of OTA in rum and walnut reveals the prospect of TrADH in food applications. The results indicate that TrADH has the potential in OTA bio-detoxification in food and feed industry. | ||
| - | + | A thermostable OTA-detoxifying hydrolase from Thermonema rossianum: identification, characterization, structure, catalytic mechanism, and application.,Xu N, Yan M, Liang X, Qin H, Gao J, Liu W Food Chem. 2025 Sep 1;485:144515. doi: 10.1016/j.foodchem.2025.144515. Epub 2025 , Apr 25. PMID:40318335<ref>PMID:40318335</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9kov" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: Wu | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Pyrinomonas methylaliphatogenes]] |
| + | [[Category: Chen YY]] | ||
| + | [[Category: Han X]] | ||
| + | [[Category: Jian G]] | ||
| + | [[Category: Liu W]] | ||
| + | [[Category: Wei HL]] | ||
| + | [[Category: Wu P]] | ||
| + | [[Category: Xu NN]] | ||
Current revision
Crystal structure of an amidohydrolase mutant from Thermonema
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Categories: Large Structures | Pyrinomonas methylaliphatogenes | Chen YY | Han X | Jian G | Liu W | Wei HL | Wu P | Xu NN
