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| - | The paper investigates the molecular mechanism by which the response regulator **PhoP** | + | The paper investigates the molecular mechanism by which the response regulator **PhoP** recognises specific promoter sequences in *Mycobacterium tuberculosis* (Mtb). PhoP is a key transcriptional regulator controlling virulence-associated pathways, including lipid biosynthesis and cell-wall remodelling<Structure load='<Structure load='Insert PDB code or filename here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' /><Structure load='Insert PDB code or filen<Structure load='3r0j' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />ame here' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />. The study presents the crystal structure of the **PhoP DNA-binding domain bound to a cognate DNA duplex**, revealing how the protein achieves sequence-specific recognition through its helix–turn–helix (HTH) motif. This insight explains how PhoP precisely regulates virulence genes crucial for Mtb survival within host environments. |
'''PDB DOI:''' https://doi.org/10.2210/pdb3R0J/pdb | '''PDB DOI:''' https://doi.org/10.2210/pdb3R0J/pdb | ||
Revision as of 12:55, 30 November 2025
Structural Basis of DNA Recognition by PhoP from *Mycobacterium tuberculosis* (PDB ID: 3R0J)
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PDB DOI: https://doi.org/10.2210/pdb3R0J/pdb Classification: Transcription regulator, DNA-binding protein Organism(s): *Mycobacterium tuberculosis* Expression System: *Escherichia coli* Membrane Protein: No Deposition Authors: (add paper authors here)
Contents |
Experimental Snapshot
• **Method Used:** X-ray crystallography • **Resolution:** 1.90 Å • **Complex Studied:** PhoP DNA-binding domain + promoter DNA • **Oligomeric State:** Symmetric dimer • **Biological Role:** Regulation of virulence genes in Mtb
Introduction: The PhoP Regulatory System
• PhoP is the response regulator of the two-component system PhoP/PhoR. • It controls lipid biosynthesis, secretion systems, and virulence genes. • The 3R0J structure reveals the core mechanism of **DNA sequence selectivity**. • Understanding PhoP is important for TB pathogenesis and drug-target development.
Function and Biological Context
• **Primary Function:** Promoter binding and transcriptional regulation. • **Activation Pathway:** PhoP becomes activated when phosphorylated by its sensor kinase PhoR. • **Importance:** Shapes gene expression programs needed for survival under host immune stress. • **Mutational Evidence:** Loss-of-function mutations impair virulence in TB models.
Structure of the PhoP–DNA Complex (3R0J)
Total Structure Overview: The PhoP DNA-binding domain forms a **dimer**, with each monomer inserting a helix–turn–helix (HTH) motif into the major groove of the DNA.
Recognition Helix (α3): • Inserts directly into the major groove. • Forms base-specific hydrogen bonds with conserved nucleotides. • Provides most of the sequence specificity.
Wing Domain (β-hairpin): • Extends toward the minor groove. • Stabilizes DNA binding through electrostatic interactions.
Key Residues Identified: • Arginine and lysine residues contact guanine and adenine bases. • Mutational studies confirm their essential role in binding.
DNA Contacting Residues
• Major groove recognition: Arg###, Lys###, Glu### (insert actual numbers). • Minor groove stabilization: Thr###, Ser###. • Dimer interface residues maintain proper spacing of HTH motifs.
Mechanism of DNA Sequence Recognition
• PhoP binds to a consensus promoter sequence known as the **PhoP box**. • Specific hydrogen-bonding pairs determine target-gene selectivity. • Dimerization increases DNA-binding affinity and promoter specificity. • Structural comparisons reveal conservation among OmpR-family regulators.
Relevance to Mycobacterial Virulence
• PhoP regulates lipid biosynthesis genes within the Mtb cell envelope. • Necessary for virulence in macrophage and animal models. • Explains why PhoP mutations lead to attenuation. • Structural insight supports therapeutic strategies targeting DNA-binding regulators.
Conclusion
The structure of PhoP bound to its target DNA reveals a detailed molecular mechanism of promoter recognition. Sequence-specific contacts mediated by the recognition helix and wing domain enable PhoP to precisely regulate transcription of virulence-associated genes in *M. tuberculosis*. This work deepens our understanding of bacterial regulatory networks and highlights PhoP as a potential target for anti-TB therapies.
References
Structural basis of DNA sequence recognition by the response regulator PhoP in Mycobacterium tuberculosis. (add full citation)
