1x6x
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1x6x.gif|left|200px]] | [[Image:1x6x.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1x6x", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1x6x| PDB=1x6x | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Structure 2: cryocolled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.95A resolution''' | '''Structure 2: cryocolled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.95A resolution''' | ||
| Line 28: | Line 25: | ||
[[Category: Hazes, B.]] | [[Category: Hazes, B.]] | ||
[[Category: Irvin, R T.]] | [[Category: Irvin, R T.]] | ||
| - | [[Category: | + | [[Category: Adhesin]] |
| - | [[Category: | + | [[Category: Lectin]] |
| - | [[Category: | + | [[Category: Type iv pilin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:38:49 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:38, 3 May 2008
Structure 2: cryocolled crystal structure of the truncated pak pilin from Pseudomonas aeruginosa at 0.95A resolution
Overview
High-resolution protein structures are becoming more common owing to the availability of increasingly brilliant synchrotron X-ray sources. However, to withstand the increased X-ray dose the crystals must be held at cryogenic temperatures. To compare the benefit of increased resolution with the drawback of potential temperature-induced changes, three room-temperature and three cryogenic data sets for PAK pilin have been collected at resolutions between 1.8 and 0.78 A. The results show that although the high-resolution cryogenic structures are more precise and more detailed, they also show systematic deviations from the room-temperature structures. Small but significant differences are even observed in the structural core, whilst more extensive changes occur at the protein surface. These differences can affect biological interpretations, especially because many important biological processes take place at the protein surface. Accordingly, although high-quality cryogenic synchrotron data is extremely valuable to protein crystallography, room-temperature structures are still desirable, especially if the research question involves protein features that are sensitive to temperature-induced changes.
About this Structure
1X6X is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Pros and cons of cryocrystallography: should we also collect a room-temperature data set?, Dunlop KV, Irvin RT, Hazes B, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):80-7. Epub 2004 Dec, 17. PMID:15608379 Page seeded by OCA on Sat May 3 14:38:49 2008
