1x7q
From Proteopedia
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'''Crystal structure of HLA-A*1101 with sars nucleocapsid peptide''' | '''Crystal structure of HLA-A*1101 with sars nucleocapsid peptide''' | ||
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[[Category: Gajhede, M.]] | [[Category: Gajhede, M.]] | ||
[[Category: Kastrup, J S.]] | [[Category: Kastrup, J S.]] | ||
| - | [[Category: | + | [[Category: Hla]] |
| - | [[Category: | + | [[Category: Human leukocyte antigen]] |
| - | [[Category: | + | [[Category: Immunoglobulin family]] |
| - | [[Category: | + | [[Category: Major histocompatibility complex class i]] |
| - | [[Category: | + | [[Category: Mhc-i]] |
| - | [[Category: | + | [[Category: Peptide-mhc-complex]] |
| - | [[Category: | + | [[Category: Sar]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:40:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:40, 3 May 2008
Crystal structure of HLA-A*1101 with sars nucleocapsid peptide
Overview
The structure of the human MHC-I molecule HLA-A*1101 in complex with a nonameric peptide (KTFPPTEPK) has been determined by X-ray crystallography to 1.45 A resolution. The peptide is derived from the SARS-CoV nucleocapsid protein positions 362-370 (SNP362-370). It is conserved in all known isolates of SARS-CoV and has been verified by in vitro peptide-binding studies to be a good to intermediate binder to HLA-A*0301 and HLA-A*1101, with IC50 values of 70 and 186 nM, respectively [Sylvester-Hvid et al. (2004), Tissue Antigens, 63, 395-400]. In terms of the residues lining the peptide-binding groove, the HLA-A*1101-SNP362-370 complex is very similar to other known structures of HLA-A*1101 and HLA-A*6801. The SNP362-370 peptide is held in place by 17 hydrogen bonds to the alpha-chain residues and by nine water molecules which are also tightly bound in the peptide-binding groove. Thr6 of the peptide (Thr6p) does not make efficient use of the middle (E) pocket. For vaccine development, there seems to be a potential for optimization targeted at this position. All residues except Thr2p and Lys9p are accessible for T-cell recognition.
About this Structure
1X7Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
High-resolution structure of HLA-A*1101 in complex with SARS nucleocapsid peptide., Blicher T, Kastrup JS, Buus S, Gajhede M, Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1031-40. Epub 2005, Jul 20. PMID:16041067 Page seeded by OCA on Sat May 3 14:40:30 2008
