1x7u

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[[Image:1x7u.jpg|left|200px]]
[[Image:1x7u.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1x7u |SIZE=350|CAPTION= <scene name='initialview01'>1x7u</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1x7u", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= KatG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28450 Burkholderia pseudomallei])
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-->
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|DOMAIN=
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{{STRUCTURE_1x7u| PDB=1x7u | SCENE= }}
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|RELATEDENTRY=[[1mwv|1MWV]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7u OCA], [http://www.ebi.ac.uk/pdbsum/1x7u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x7u RCSB]</span>
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}}
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'''Crystal structure of the S324T of catalase-peroxidase KatG'''
'''Crystal structure of the S324T of catalase-peroxidase KatG'''
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[[Category: Singh, R.]]
[[Category: Singh, R.]]
[[Category: Wiseman, B.]]
[[Category: Wiseman, B.]]
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[[Category: catalase-peroxidase]]
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[[Category: Catalase-peroxidase]]
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[[Category: inh resistant variant]]
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[[Category: Inh resistant variant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:40:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:45:53 2008''
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Revision as of 11:40, 3 May 2008

Image:1x7u.jpg

Template:STRUCTURE 1x7u

Crystal structure of the S324T of catalase-peroxidase KatG


Overview

The Ser315Thr variant of the catalase-peroxidase KatG from Mycobacterium tuberculosis imparts resistance to the pro-drug isonicotinic acid hydrazide (isoniazid) through a failure to convert it to the active drug, isonicotinoyl-NAD. The equivalent variant in KatG from Burkholderia pseudomallei, Ser324Thr, has been constructed, revealing catalase and peroxidase activities that are similar to those of the native enzyme. The other activities of the variant protein, including the NADH oxidase, the isoniazid hydrazinolysis and isonicotinoyl-NAD synthase activities are reduced by 60-70%. The crystal structure of the variant differs from that of the native enzyme in having the methyl group of Thr324 situated in the entrance channel to the heme cavity, in a modified water matrix in the entrance channel and heme cavity, in lacking the putative perhydroxy modification on the heme, in the multiple locations of a few side-chains, and in the presence of an apparent perhydroxy modification on the indole nitrogen atom of the active-site Trp111. The position of the methyl group of Thr324 creates a constriction or narrowing of the channel leading to the heme cavity, providing an explanation for the lower reactivity towards isoniazid and the slower rate of isonicotinoyl-NAD synthesis.

About this Structure

1X7U is a Single protein structure of sequence from Burkholderia pseudomallei. Full crystallographic information is available from OCA.

Reference

Structural characterization of the Ser324Thr variant of the catalase-peroxidase (KatG) from Burkholderia pseudomallei., Deemagarn T, Carpena X, Singh R, Wiseman B, Fita I, Loewen PC, J Mol Biol. 2005 Jan 7;345(1):21-8. PMID:15567407 Page seeded by OCA on Sat May 3 14:40:48 2008

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