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9igw
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Ku70/80 bound to 147 bp nucleosome== | |
| + | <StructureSection load='9igw' size='340' side='right'caption='[[9igw]], [[Resolution|resolution]] 3.32Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9igw]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IGW FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.32Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9igw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9igw OCA], [https://pdbe.org/9igw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9igw RCSB], [https://www.ebi.ac.uk/pdbsum/9igw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9igw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/H31_HUMAN H31_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA double-strand breaks (DSBs) are highly deleterious lesions that can trigger cell death or carcinogenesis if unrepaired or misrepaired. In mammals, most DSBs are repaired by non-homologous end joining (NHEJ), which begins when Ku70/80 binds DNA ends and recruits DNA-PKcs to form the DNA-PK holoenzyme. Although recent cryo-EM studies have resolved several NHEJ assemblies, how these factors access DSBs within nucleosomes remains unclear. Here, we present cryo-EM structures of human Ku70/80 and DNA-PK bound to nucleosomes. Ku70/80 binds the DNA end and bends it away from the nucleosome core, while the Ku70 C-terminal SAP domain makes an additional, specific DNA contact. Our DNA-PK-nucleosome structure further reveals the opening of the Ku80 vWA domain, and we show that non-hydrolysable ATP promotes synapsis by stabilising the Ku80-mediated DNA-PK dimer. These structures reveal a model for DSB recognition on nucleosomal DNA and provide insights relevant to targeting NHEJ in cancer therapy. | ||
| - | + | Cryo-EM structures of NHEJ assemblies with nucleosomes.,Hall C, Frit P, Kefala-Stavridi A, Pelletier A, Hardwick SW, Amin H, Bilyard MK, Maia De Oliviera T, Tariq A, Zahid S, Chirgadze DY, Balasubramanian S, Meek K, Ropars V, Charbonnier JB, Modesti M, Calsou P, Britton S, Blundell TL, Schalch T, Chaplin AK Nat Commun. 2025 Dec 24. doi: 10.1038/s41467-025-67376-2. PMID:41444611<ref>PMID:41444611</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Chaplin | + | <div class="pdbe-citations 9igw" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hall | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chaplin AK]] | ||
| + | [[Category: Hall C]] | ||
Current revision
Ku70/80 bound to 147 bp nucleosome
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