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1xcb

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[[Image:1xcb.jpg|left|200px]]
[[Image:1xcb.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1xcb |SIZE=350|CAPTION= <scene name='initialview01'>1xcb</scene>, resolution 2.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1xcb", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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or leave the SCENE parameter empty for the default display.
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|GENE= rex ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02629 CoA_binding], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK05472 PRK05472], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam06971 Put_DNA-bind_N]</span>
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{{STRUCTURE_1xcb| PDB=1xcb | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcb OCA], [http://www.ebi.ac.uk/pdbsum/1xcb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xcb RCSB]</span>
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'''X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus'''
'''X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus'''
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==About this Structure==
==About this Structure==
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1XCB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. This structure supersedes the now removed PDB entry 1R72. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCB OCA].
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1XCB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1r72 1r72]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCB OCA].
==Reference==
==Reference==
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[[Category: Paranawithana, S.]]
[[Category: Paranawithana, S.]]
[[Category: Sickmier, E A.]]
[[Category: Sickmier, E A.]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: new york structural genomix research consortium]]
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[[Category: New york structural genomix research consortium]]
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[[Category: nicotinamide adenine dinucleotide]]
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[[Category: Nicotinamide adenine dinucleotide]]
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[[Category: nysgxrc]]
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[[Category: Nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: psi]]
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[[Category: Psi]]
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[[Category: redox-sensing]]
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[[Category: Redox-sensing]]
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[[Category: rex]]
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[[Category: Rex]]
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[[Category: rossmann fold]]
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[[Category: Rossmann fold]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: thermus aquaticus]]
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[[Category: Thermus aquaticus]]
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[[Category: winged helix]]
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[[Category: Winged helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:50:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:47:40 2008''
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Revision as of 11:50, 3 May 2008

Image:1xcb.jpg

Template:STRUCTURE 1xcb

X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus


Overview

The redox-sensing repressor Rex regulates transcription of respiratory genes in response to the intra cellular NADH/NAD(+) redox poise. As a step toward elucidating the molecular mechanism of NADH/NAD(+) sensing, the X-ray structure of Thermus aquaticus Rex (T-Rex) bound to effector NADH has been determined at 2.9 A resolution. The fold of the C-terminal domain of T-Rex is characteristic of NAD(H)-dependent enzymes, whereas the N-terminal domain is similar to a winged helix DNA binding motif. T-Rex dimerization is primarily mediated by "domain-swapped" alpha helices. Each NADH molecule binds to the C-terminal domain near the dimer interface. In contrast to NAD(H)-dependent enzymes, the nicotinamide is deeply buried within a hydrophobic pocket that appears to preclude substrate entry. We show that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T-Rex/DNA binding activity. A mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes.

About this Structure

1XCB is a Single protein structure of sequence from Thermus aquaticus. This structure supersedes the now removed PDB entry 1r72. Full crystallographic information is available from OCA.

Reference

X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing., Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL, Structure. 2005 Jan;13(1):43-54. PMID:15642260 Page seeded by OCA on Sat May 3 14:50:50 2008

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