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1xef
From Proteopedia
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'''Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD''' | '''Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD''' | ||
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[[Category: Schmitt, L.]] | [[Category: Schmitt, L.]] | ||
[[Category: Zaitseva, J.]] | [[Category: Zaitseva, J.]] | ||
| - | [[Category: | + | [[Category: Abc-transporter]] |
| - | [[Category: | + | [[Category: Atp-dependent transport protein]] |
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Haemolysin b]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:55:20 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:55, 3 May 2008
Crystal structure of the ATP/Mg2+ bound composite dimer of HlyB-NBD
Overview
The ABC transporter HlyB is a central element of the HlyA secretion machinery, a paradigm of Type I secretion. Here, we describe the crystal structure of the HlyB-NBD (nucleotide-binding domain) with H662 replaced by Ala in complex with ATP/Mg2+. The dimer shows a composite architecture, in which two intact ATP molecules are bound at the interface of the Walker A motif and the C-loop, provided by the two monomers. ATPase measurements confirm that H662 is essential for activity. Based on these data, we propose a model in which E631 and H662, highly conserved among ABC transporters, form a catalytic dyad. Here, H662 acts as a 'linchpin', holding together all required parts of a complicated network of interactions between ATP, water molecules, Mg2+, and amino acids both in cis and trans, necessary for intermonomer communication. Based on biochemical experiments, we discuss the hypothesis that substrate-assisted catalysis, rather than general base catalysis might operate in ABC-ATPases.
About this Structure
1XEF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB., Zaitseva J, Jenewein S, Jumpertz T, Holland IB, Schmitt L, EMBO J. 2005 Jun 1;24(11):1901-10. Epub 2005 May 12. PMID:15889153 Page seeded by OCA on Sat May 3 14:55:20 2008
