1xfn
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1xfn.gif|left|200px]] | [[Image:1xfn.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1xfn", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1xfn| PDB=1xfn | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part''' | '''NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part''' | ||
| Line 34: | Line 31: | ||
[[Category: Marks, D.]] | [[Category: Marks, D.]] | ||
[[Category: Nuland, N A.van.]] | [[Category: Nuland, N A.van.]] | ||
| - | [[Category: | + | [[Category: Pas domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:58:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 11:58, 3 May 2008
NMR structure of the ground state of the photoactive yellow protein lacking the N-terminal part
Overview
The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.
About this Structure
1XFN is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein., Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA, Structure. 2005 Jul;13(7):953-62. PMID:16004868 Page seeded by OCA on Sat May 3 14:58:03 2008
