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1xjv
From Proteopedia
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'''Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)''' | '''Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)''' | ||
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[[Category: Lei, M.]] | [[Category: Lei, M.]] | ||
[[Category: Podell, E R.]] | [[Category: Podell, E R.]] | ||
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| - | [[Category: | + | [[Category: Single-stranded dna]] |
| - | [[Category: | + | [[Category: Telomere]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:07:22 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:07, 3 May 2008
Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)
Overview
The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. Here, we report the crystal structure at a resolution of 1.73 A of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays. The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB) folds; the N-terminal OB fold binds the first six nucleotides, resembling the structure of the S. pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping.
About this Structure
1XJV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection., Lei M, Podell ER, Cech TR, Nat Struct Mol Biol. 2004 Dec;11(12):1223-9. Epub 2004 Nov 21. PMID:15558049 Page seeded by OCA on Sat May 3 15:07:22 2008
