1xn6

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[[Image:1xn6.jpg|left|200px]]
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{{Structure
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG3832 COG3832]</span>
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{{STRUCTURE_1xn6| PDB=1xn6 | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xn6 OCA], [http://www.ebi.ac.uk/pdbsum/1xn6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xn6 RCSB]</span>
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'''Solution Structure of Northeast Structural Genomics Target Protein BcR68 encoded in gene Q816V6 of B. cereus'''
'''Solution Structure of Northeast Structural Genomics Target Protein BcR68 encoded in gene Q816V6 of B. cereus'''
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[[Category: Xiao, R.]]
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[[Category: nesg target protein bcr68]]
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[[Category: Nesg target protein bcr68]]
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[[Category: northeast structural genomics consortium]]
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[[Category: Northeast structural genomics consortium]]
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[[Category: protein structure initiative]]
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Revision as of 12:15, 3 May 2008

Image:1xn6.jpg

Template:STRUCTURE 1xn6

Solution Structure of Northeast Structural Genomics Target Protein BcR68 encoded in gene Q816V6 of B. cereus


Overview

A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform NMR experiments for resonance assignment based on highly resolved 4D and 5D spectral information is acquired in conjunction with a single simultaneous 3D 15N,13C(aliphatic),13C(aromatic)-resolved [1H,1H]-NOESY spectrum providing 1H-1H upper distance limit constraints. The protocol was integrated with methodology for semiautomated data analysis and used to solve eight NMR protein structures of the Northeast Structural Genomics Consortium pipeline. The molecular masses of the hypothetical target proteins ranged from 9 to 20 kDa with an average of approximately 14 kDa. Between 1 and 9 days of instrument time were invested per structure, which is less than approximately 10-25% of the measurement time routinely required to date with conventional approaches. The protocol presented here effectively removes data collection as a bottleneck for high-throughput solution structure determination of proteins up to at least approximately 20 kDa, while concurrently providing spectra that are highly amenable to fast and robust analysis.

About this Structure

1XN6 is a Single protein structure of sequence from Bacillus cereus. Full crystallographic information is available from OCA.

Reference

NMR data collection and analysis protocol for high-throughput protein structure determination., Liu G, Shen Y, Atreya HS, Parish D, Shao Y, Sukumaran DK, Xiao R, Yee A, Lemak A, Bhattacharya A, Acton TA, Arrowsmith CH, Montelione GT, Szyperski T, Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10487-92. Epub 2005 Jul 18. PMID:16027363 Page seeded by OCA on Sat May 3 15:15:05 2008

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OCA

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