1xs9

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[[Image:1xs9.gif|left|200px]]
[[Image:1xs9.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1xs9 |SIZE=350|CAPTION= <scene name='initialview01'>1xs9</scene>
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The line below this paragraph, containing "STRUCTURE_1xs9", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= marA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), rpoA, pez, phs, sez ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1xs9| PDB=1xs9 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xs9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xs9 OCA], [http://www.ebi.ac.uk/pdbsum/1xs9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xs9 RCSB]</span>
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}}
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'''A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA'''
'''A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA'''
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==About this Structure==
==About this Structure==
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1XS9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1TI9. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS9 OCA].
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1XS9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ti9 1ti9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XS9 OCA].
==Reference==
==Reference==
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[[Category: Martin, R G.]]
[[Category: Martin, R G.]]
[[Category: Rosner, J L.]]
[[Category: Rosner, J L.]]
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[[Category: mara]]
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[[Category: Mara]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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[[Category: rna polymerase]]
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[[Category: Rna polymerase]]
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[[Category: ternary complex]]
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[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:26:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:53:59 2008''
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Revision as of 12:26, 3 May 2008

Image:1xs9.gif

Template:STRUCTURE 1xs9

A MODEL OF THE TERNARY COMPLEX FORMED BETWEEN MARA, THE ALPHA-CTD OF RNA POLYMERASE AND DNA


Overview

The transcriptional activator, MarA, interacts with RNA polymerase (RNAP) to activate promoters of the mar regulon. Here, we identify the interacting surfaces of MarA and of the carboxy-terminal domain of the alpha subunit of RNAP (alpha-CTD) by NMR-based chemical shift mapping. Spectral changes were monitored for a MarA-DNA complex upon titration with alpha-CTD, and for alpha-CTD upon titration with MarA-DNA. The mapping results were confirmed by mutational studies and retention chromatography. A model of the ternary complex shows that alpha-CTD uses a '265-like determinant' to contact MarA at a surface distant from the DNA. This is unlike the interaction of alpha-CTD with the CRP or Fis activators where the '265 determinant' contacts DNA while another surface of the same alpha-CTD molecule contacts the activator. These results reveal a new versatility for alpha-CTD in transcriptional activation.

About this Structure

1XS9 is a Protein complex structure of sequences from Escherichia coli. This structure supersedes the now removed PDB entry 1ti9. Full crystallographic information is available from OCA.

Reference

Versatility of the carboxy-terminal domain of the alpha subunit of RNA polymerase in transcriptional activation: use of the DNA contact site as a protein contact site for MarA., Dangi B, Gronenborn AM, Rosner JL, Martin RG, Mol Microbiol. 2004 Oct;54(1):45-59. PMID:15458404 Page seeded by OCA on Sat May 3 15:26:53 2008

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