1xxs
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1xxs.gif|left|200px]] | [[Image:1xxs.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1xxs", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1xxs| PDB=1xxs | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structural insights for fatty acid binding in a Lys49 phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid''' | '''Structural insights for fatty acid binding in a Lys49 phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid''' | ||
| Line 24: | Line 21: | ||
Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid., Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK, Biochimie. 2005 Feb;87(2):161-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15760708 15760708] | Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid., Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK, Biochimie. 2005 Feb;87(2):161-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15760708 15760708] | ||
[[Category: Bothrops moojeni]] | [[Category: Bothrops moojeni]] | ||
| - | [[Category: Phospholipase A(2)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arni, R K.]] | [[Category: Arni, R K.]] | ||
| Line 31: | Line 27: | ||
[[Category: Ward, R J.]] | [[Category: Ward, R J.]] | ||
[[Category: Watanabe, L.]] | [[Category: Watanabe, L.]] | ||
| - | [[Category: | + | [[Category: Crystal structure]] |
| - | [[Category: | + | [[Category: Dimer interface]] |
| - | [[Category: | + | [[Category: Fatty acid binding]] |
| - | [[Category: | + | [[Category: Phospholipase a2]] |
| - | [[Category: | + | [[Category: Stearic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:38:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 12:38, 3 May 2008
Structural insights for fatty acid binding in a Lys49 phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid
Overview
The crystal structure of dimeric Lys49-phospholipase A2 myotoxin-II from Bothrops moojeni (MjTX-II) co-crystallized with stearic acid (C(18)H(36)O(2)) has been determined at a resolution of 1.8 A. The electron density maps permitted the unambiguous inclusion of six stearic acid molecules in the refinement. Two stearic acid molecules could be located in the substrate-binding cleft of each monomer in positions, which favor the interaction of their carboxyl groups with active site residues. The way of binding of stearic acids to this Lys49-PLA(2)s is analogous to phospholipids and transition state analogues to catalytically active PLA(2)s. Two additional stearic acid molecules were located at the dimer interface region, defining a hitherto unidentified acyl-binding site on the protein surface. The strictly conserved Lys122 for Lys49-PLA(2)s may play a fundamental role for stabilization of legend-protein complex. The comparison of MjTX-II/satiric acid complex with other Lys-PLA(2)s structures whose putative fatty acids were located at their active site is also analysed. Molecular details of the stearic acid/protein interactions provide insights to binding in group I/II PLA(2)s, and to the possible interactions of Lys49-PLA(2)s with target membranes.
About this Structure
1XXS is a Single protein structure of sequence from Bothrops moojeni. Full crystallographic information is available from OCA.
Reference
Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid., Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK, Biochimie. 2005 Feb;87(2):161-7. PMID:15760708 Page seeded by OCA on Sat May 3 15:38:37 2008
