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1xxu

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[[Image:1xxu.gif|left|200px]]
[[Image:1xxu.gif|left|200px]]
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{{Structure
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|PDB= 1xxu |SIZE=350|CAPTION= <scene name='initialview01'>1xxu</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_1xxu", creates the "Structure Box" on the page.
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|SITE=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] </span>
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|GENE= Rv2238c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd03018 PRX_AhpE_like]</span>
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{{STRUCTURE_1xxu| PDB=1xxu | SCENE= }}
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|RELATEDENTRY=[[1xvw|1xvw]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxu OCA], [http://www.ebi.ac.uk/pdbsum/1xxu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xxu RCSB]</span>
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'''Crystal Structure of AhpE from Mycrobacterium tuberculosis, a 1-Cys peroxiredoxin'''
'''Crystal Structure of AhpE from Mycrobacterium tuberculosis, a 1-Cys peroxiredoxin'''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:38:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:56:05 2008''
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Revision as of 12:38, 3 May 2008

Image:1xxu.gif

Template:STRUCTURE 1xxu

Crystal Structure of AhpE from Mycrobacterium tuberculosis, a 1-Cys peroxiredoxin


Overview

All living systems require protection against the damaging effects of reactive oxygen species. The genome of Mycobacterium tuberculosis, the cause of TB, encodes a number of peroxidases that are thought to be active against organic and inorganic peroxides, and are likely to play a key role in the ability of this organism to survive within the phagosomes of macrophages. The open reading frame Rv2238c in M.tuberculosis encodes a 153-residue protein AhpE, which is a peroxidase of the 1-Cys peroxiredoxin (Prx) family. The crystal structure of AhpE, determined at 1.87 A resolution (R(cryst)=0.179, R(free)=0.210), reveals a compact single-domain protein with a thioredoxin fold. AhpE forms both dimers and octamers; a tightly-associated dimer and a ring-like octamer, generated by crystallographic 4-fold symmetry. In this native structure, the active site Cys45 is in its oxidized, sulfenic acid (S-O-H) state. A second crystal form of AhpE, obtained after soaking in sodium bromide and refined at 1.90 A resolution (R(cryst)=0.242, R(free)=0.286), reveals the reduced structure. In this structure, a conformational change in an external loop, in two of the four molecules in the asymmetric unit, allows Arg116 to stabilise the Cys45 thiolate ion, and concomitantly closes a surface channel. This channel is identified as the likely binding site for a physiological reductant, and the conformational change is inferred to be important for the reaction cycle of AhpE.

About this Structure

1XXU is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin., Li S, Peterson NA, Kim MY, Kim CY, Hung LW, Yu M, Lekin T, Segelke BW, Lott JS, Baker EN, J Mol Biol. 2005 Mar 4;346(4):1035-46. Epub 2005 Jan 25. PMID:15701515 Page seeded by OCA on Sat May 3 15:38:40 2008

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