1y0v

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[[Image:1y0v.gif|left|200px]]
[[Image:1y0v.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1y0v |SIZE=350|CAPTION= <scene name='initialview01'>1y0v</scene>, resolution 3.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1y0v", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= cya ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), CALM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis])
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-->
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|DOMAIN=
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{{STRUCTURE_1y0v| PDB=1y0v | SCENE= }}
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|RELATEDENTRY=[[1xfv|1XFV]], [[1xfw|1XFW]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0v OCA], [http://www.ebi.ac.uk/pdbsum/1y0v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y0v RCSB]</span>
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}}
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'''Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate'''
'''Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate'''
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[[Category: Tang, W J.]]
[[Category: Tang, W J.]]
[[Category: Zhukovskaya, N L.]]
[[Category: Zhukovskaya, N L.]]
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[[Category: anthrax edema factor]]
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[[Category: Anthrax edema factor]]
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[[Category: calcium-independent]]
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[[Category: Calcium-independent]]
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[[Category: calmodulin]]
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[[Category: Calmodulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:44:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:57:18 2008''
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Revision as of 12:44, 3 May 2008

Image:1y0v.gif

Template:STRUCTURE 1y0v

Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate


Overview

Edema factor (EF), a key anthrax exotoxin, has an anthrax protective antigen-binding domain (PABD) and a calmodulin (CaM)-activated adenylyl cyclase domain. Here, we report the crystal structures of CaM-bound EF, revealing the architecture of EF PABD. CaM has N- and C-terminal domains and each domain can bind two calcium ions. Calcium binding induces the conformational change of CaM from closed to open. Structures of the EF-CaM complex show how EF locks the N-terminal domain of CaM into a closed conformation regardless of its calcium-loading state. This represents a mechanism of how CaM effector alters the calcium affinity of CaM and uncouples the conformational change of CaM from calcium loading. Furthermore, structures of EF-CaM complexed with nucleotides show that EF uses two-metal-ion catalysis, a prevalent mechanism in DNA and RNA polymerases. A histidine (H351) further facilitates the catalysis of EF by activating a water to deprotonate 3'OH of ATP. Mammalian adenylyl cyclases share no structural similarity with EF and they also use two-metal-ion catalysis, suggesting the catalytic mechanism-driven convergent evolution of two structurally diverse adenylyl cyclases.

About this Structure

1Y0V is a Protein complex structure of sequences from Bacillus anthracis and Xenopus laevis. Full crystallographic information is available from OCA.

Reference

Calcium-independent calmodulin binding and two-metal-ion catalytic mechanism of anthrax edema factor., Shen Y, Zhukovskaya NL, Guo Q, Florian J, Tang WJ, EMBO J. 2005 Mar 9;24(5):929-41. Epub 2005 Feb 17. PMID:15719022 Page seeded by OCA on Sat May 3 15:44:55 2008

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