We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1y42

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1y42.gif|left|200px]]
[[Image:1y42.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1y42 |SIZE=350|CAPTION= <scene name='initialview01'>1y42</scene>, resolution 1.95&Aring;
+
The line below this paragraph, containing "STRUCTURE_1y42", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=TYR:TYROSINE'>TYR</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= cyt-18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1y42| PDB=1y42 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y42 OCA], [http://www.ebi.ac.uk/pdbsum/1y42 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y42 RCSB]</span>
+
-
}}
+
'''Crystal structure of a C-terminally truncated CYT-18 protein'''
'''Crystal structure of a C-terminally truncated CYT-18 protein'''
Line 33: Line 30:
[[Category: Paukstelis, P J.]]
[[Category: Paukstelis, P J.]]
[[Category: Robertus, J.]]
[[Category: Robertus, J.]]
-
[[Category: cyt-18]]
+
[[Category: Cyt-18]]
-
[[Category: group i intron]]
+
[[Category: Group i intron]]
-
[[Category: trna ligase]]
+
[[Category: Trna ligase]]
-
[[Category: tyrosyl trna synthetase]]
+
[[Category: Tyrosyl trna synthetase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:51:07 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:29 2008''
+

Revision as of 12:51, 3 May 2008

Image:1y42.gif

Template:STRUCTURE 1y42

Crystal structure of a C-terminally truncated CYT-18 protein


Overview

We determined a 1.95 A X-ray crystal structure of a C-terminally truncated Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) that functions in splicing group I introns. CYT-18's nucleotide binding fold and intermediate alpha-helical domains superimpose on those of bacterial TyrRSs, except for an N-terminal extension and two small insertions not found in nonsplicing bacterial enzymes. These additions surround the cyt-18-1 mutation site and are sites of suppressor mutations that restore splicing, but not synthetase activity. Highly constrained models based on directed hydroxyl radical cleavage assays show that the group I intron binds at a site formed in part by the three additions on the nucleotide binding fold surface opposite that which binds tRNATyr. Our results show how essential proteins can progressively evolve new functions.

About this Structure

1Y42 is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.

Reference

A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM, Mol Cell. 2005 Feb 4;17(3):417-28. PMID:15694342 Page seeded by OCA on Sat May 3 15:51:07 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y42"
Personal tools