1y44

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1y44.gif|left|200px]]
[[Image:1y44.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1y44 |SIZE=350|CAPTION= <scene name='initialview01'>1y44</scene>, resolution 2.10&Aring;
+
The line below this paragraph, containing "STRUCTURE_1y44", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= rnz ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1y44| PDB=1y44 | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y44 OCA], [http://www.ebi.ac.uk/pdbsum/1y44 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y44 RCSB]</span>
+
-
}}
+
'''Crystal structure of RNase Z'''
'''Crystal structure of RNase Z'''
Line 29: Line 26:
[[Category: Pellegrini, O.]]
[[Category: Pellegrini, O.]]
[[Category: Sierra-Gallay, I L.de la.]]
[[Category: Sierra-Gallay, I L.de la.]]
-
[[Category: zinc-dependent metal hydrolase]]
+
[[Category: Zinc-dependent metal hydrolase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:51:17 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:31 2008''
+

Revision as of 12:51, 3 May 2008

Image:1y44.gif

Template:STRUCTURE 1y44

Crystal structure of RNase Z


Overview

Transfer RNAs (tRNAs) are synthesized as part of longer primary transcripts that require processing of both their 3' and 5' extremities in every living organism known. The 5' side is processed (matured) by the ubiquitously conserved endonucleolytic ribozyme, RNase P, whereas removal of the 3' tails can be either exonucleolytic or endonucleolytic. The endonucleolytic pathway is catalysed by an enzyme known as RNase Z, or 3' tRNase. RNase Z cleaves precursor tRNAs immediately after the discriminator base (the unpaired nucleotide 3' to the last base pair of the acceptor stem, used as an identity determinant by many aminoacyl-tRNA synthetases) in most cases, yielding a tRNA primed for addition of the CCA motif by nucleotidyl transferase. Here we report the crystal structure of Bacillus subtilis RNase Z at 2.1 A resolution, and propose a mechanism for tRNA recognition and cleavage. The structure explains the allosteric properties of the enzyme, and also sheds light on the mechanisms of inhibition by the CCA motif and long 5' extensions. Finally, it highlights the extraordinary adaptability of the metallo-hydrolase domain of the beta-lactamase family for the hydrolysis of covalent bonds.

About this Structure

1Y44 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural basis for substrate binding, cleavage and allostery in the tRNA maturase RNase Z., de la Sierra-Gallay IL, Pellegrini O, Condon C, Nature. 2005 Feb 10;433(7026):657-61. Epub 2005 Jan 16. PMID:15654328 Page seeded by OCA on Sat May 3 15:51:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y44"
Personal tools