2bdn

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Revision as of 18:55, 12 November 2007

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spinqualitylabelsall models 2bdn, resolution 2.5300Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of human MCP-1 bound to a blocking antibody, 11K2

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Monocyte chemoattractant proteins (MCPs) are cytokines that direct immune, cells bearing appropriate receptors to sites of inflammation or injury and, are therefore attractive therapeutic targets for inhibitory molecules., 11K2 is a blocking mouse monoclonal antibody active against several human, and murine MCPs. A 2.5 A structure of the Fab fragment of this antibody in, complex with human MCP-1 has been solved. The Fab blocks CCR2 receptor, binding to MCP-1 through an adjacent but distinct binding site. The, orientation of the Fab indicates that a single MCP-1 dimer will bind two, 11K2 antibodies. Several key residues on the antibody and on human MCPs, were predicted to be involved in antibody selectivity. Mutational analysis, of these residues confirms their involvement in the antibody-chemokine, interaction. In addition to mutations that decreased or disrupted binding, one antibody mutation resulted in a 70-fold increase in affinity for human, MCP-2. A key residue missing in human MCP-3, a chemokine not recognized by, the antibody, was identified and engineering the preferred residue into, the chemokine conferred binding to the antibody.

Disease

Known diseases associated with this structure: Coronary artery disease, modifier of OMIM:[158105], HIV-1, resistance to OMIM:[158105], Mycobacterium tuberculosis, susceptibility to OMIM:[158105], Spina bifida, susceptiblity to OMIM:[158105]

About this Structure

2BDN is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure activity relationships of monocyte chemoattractant proteins in complex with a blocking antibody., Reid C, Rushe M, Jarpe M, van Vlijmen H, Dolinski B, Qian F, Cachero TG, Cuervo H, Yanachkova M, Nwankwo C, Wang X, Etienne N, Garber E, Bailly V, de Fougerolles A, Boriack-Sjodin PA, Protein Eng Des Sel. 2006 Jul;19(7):317-24. Epub 2006 May 8. PMID:16682434

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