1y4z

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[[Image:1y4z.gif|left|200px]]
[[Image:1y4z.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1y4z |SIZE=350|CAPTION= <scene name='initialview01'>1y4z</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_1y4z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=6MO:MOLYBDENUM(VI)+ION'>6MO</scene>, <scene name='pdbligand=AGA:(1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL+OCTANOATE'>AGA</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MD1:PHOSPHORIC+ACID+4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL+ESTER+GUANYLATE+ESTER'>MD1</scene>, <scene name='pdbligand=PCI:PENTACHLOROPHENOL'>PCI</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= narG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), narH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), narI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1y4z| PDB=1y4z | SCENE= }}
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|RELATEDENTRY=[[1q16|1Q16]], [[1siw|1SIW]], [[1y5i|1Y5I]], [[1y5l|1Y5L]], [[1y5n|1Y5N]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4z OCA], [http://www.ebi.ac.uk/pdbsum/1y4z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y4z RCSB]</span>
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}}
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'''The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol'''
'''The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol'''
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[[Category: Strynadka, N C.J.]]
[[Category: Strynadka, N C.J.]]
[[Category: Weiner, J H.]]
[[Category: Weiner, J H.]]
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[[Category: electron transfer]]
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[[Category: Electron transfer]]
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[[Category: membrane protein]]
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[[Category: Membrane protein]]
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[[Category: nitrate reduction]]
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[[Category: Nitrate reduction]]
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[[Category: q-site]]
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[[Category: Q-site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:53:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:58:54 2008''
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Revision as of 12:53, 3 May 2008

Image:1y4z.gif

Template:STRUCTURE 1y4z

The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol


Overview

The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.

About this Structure

1Y4Z is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A., Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC, J Biol Chem. 2005 Apr 15;280(15):14836-43. Epub 2004 Dec 22. PMID:15615728 Page seeded by OCA on Sat May 3 15:53:02 2008

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