1y8m
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1y8m.gif|left|200px]] | [[Image:1y8m.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1y8m", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1y8m| PDB=1y8m | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Solution Structure of Yeast Mitochondria Fission Protein Fis1''' | '''Solution Structure of Yeast Mitochondria Fission Protein Fis1''' | ||
| Line 28: | Line 25: | ||
[[Category: Tjandra, N.]] | [[Category: Tjandra, N.]] | ||
[[Category: Youle, R J.]] | [[Category: Youle, R J.]] | ||
| - | [[Category: | + | [[Category: Fission]] |
| - | [[Category: | + | [[Category: Mitochondria]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:00:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:00, 3 May 2008
Solution Structure of Yeast Mitochondria Fission Protein Fis1
Overview
Mitochondrial fission is facilitated by a multiprotein complex assembled at the division site. The required components of the fission machinery in Saccharomyces cerevisiae include Dnm1, Fis1, and Mdv1. In the present study, we determined the protein structure of yeast Fis1 using NMR spectroscopy. Although the six alpha-helices, as well as their folding, in the yeast Fis1 structure are similar to those of the tetratricopeptide repeat (TPR) domains of the human Fis1 structure, the two structures differ in their N termini. The N-terminal tail of human Fis1 is flexible and unstructured, whereas a major segment of the longer N terminus of yeast Fis1 is fixed to the concave face formed by the six alpha-helices in the TPR domains. To investigate the role of the fixed N terminus, exogenous Fis1 was expressed in yeast lacking the endogenous protein. Expression of yeast Fis1 protein rescued mitochondrial fission in delta fis1 yeast only when the N-terminal TPR binding segment was left intact. The presence of this segment is also correlated to the recruitment of Mdv1 to mitochondria. The conformation of the N-terminal segment embedded in the TPR pocket indicates an intra-molecular regulation of Fis1 bioactivity. Although the TPR-like helix bundle of Fis1 mediates the interaction with Dnm1 and Mdv1, the N terminus of Fis1 is a prerequisite to recruit Mdv1 to facilitate mitochondrial fission.
About this Structure
1Y8M is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Novel structure of the N terminus in yeast Fis1 correlates with a specialized function in mitochondrial fission., Suzuki M, Neutzner A, Tjandra N, Youle RJ, J Biol Chem. 2005 Jun 3;280(22):21444-52. Epub 2005 Apr 4. PMID:15809300 Page seeded by OCA on Sat May 3 16:00:42 2008
