1y8r

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[[Image:1y8r.gif|left|200px]]
[[Image:1y8r.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1y8r |SIZE=350|CAPTION= <scene name='initialview01'>1y8r</scene>, resolution 2.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1y8r", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= UBLE1A, SAE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBLE1B, SAE2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBL1, SUMO-1, SMT3C, SMT3H3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1y8r| PDB=1y8r | SCENE= }}
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|RELATEDENTRY=[[1y8q|1Y8Q]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y8r OCA], [http://www.ebi.ac.uk/pdbsum/1y8r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y8r RCSB]</span>
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}}
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'''SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX'''
'''SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX'''
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[[Category: Lima, C D.]]
[[Category: Lima, C D.]]
[[Category: Lois, L M.]]
[[Category: Lois, L M.]]
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[[Category: activating enzyme]]
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[[Category: Activating enzyme]]
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[[Category: e1]]
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[[Category: E1]]
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[[Category: heterodimer]]
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[[Category: Heterodimer]]
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[[Category: sumo]]
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[[Category: Sumo]]
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[[Category: ubl]]
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[[Category: Ubl]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:00:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:00:15 2008''
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Revision as of 13:00, 3 May 2008

Image:1y8r.gif

Template:STRUCTURE 1y8r

SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX


Overview

E1 enzymes facilitate conjugation of ubiquitin and ubiquitin-like proteins through adenylation, thioester transfer within E1, and thioester transfer from E1 to E2 conjugating proteins. Structures of human heterodimeric Sae1/Sae2-Mg.ATP and Sae1/Sae2-SUMO-1-Mg.ATP complexes were determined at 2.2 and 2.75 A resolution, respectively. Despite the presence of Mg.ATP, the Sae1/Sae2-SUMO-1-Mg.ATP structure reveals a substrate complex insomuch as the SUMO C-terminus remains unmodified within the adenylation site and 35 A from the catalytic cysteine, suggesting that additional changes within the adenylation site may be required to facilitate chemistry prior to adenylation and thioester transfer. A mechanism for E2 recruitment to E1 is suggested by biochemical and genetic data, each of which supports a direct role for the E1 C-terminal ubiquitin-like domain for E2 recruitment during conjugation.

About this Structure

1Y8R is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1., Lois LM, Lima CD, EMBO J. 2005 Feb 9;24(3):439-51. Epub 2005 Jan 20. PMID:15660128 Page seeded by OCA on Sat May 3 16:00:57 2008

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OCA

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