1y8x

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[[Image:1y8x.gif|left|200px]]
[[Image:1y8x.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1y8x |SIZE=350|CAPTION= <scene name='initialview01'>1y8x</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_1y8x", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= UBE2M, UBC12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBE1C, UBA3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1y8x| PDB=1y8x | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y8x OCA], [http://www.ebi.ac.uk/pdbsum/1y8x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y8x RCSB]</span>
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}}
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'''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1'''
'''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1'''
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[[Category: Waddell, M B.]]
[[Category: Waddell, M B.]]
[[Category: Zhuang, M.]]
[[Category: Zhuang, M.]]
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[[Category: ligase]]
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[[Category: Ligase]]
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[[Category: ubiquitin-conjugating enzyme e2 m]]
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[[Category: Ubiquitin-conjugating enzyme e2 m]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:01:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:00:21 2008''
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Revision as of 13:01, 3 May 2008

Image:1y8x.gif

Template:STRUCTURE 1y8x

Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1


Overview

E2 conjugating enzymes play a central role in ubiquitin and ubiquitin-like protein (ublp) transfer cascades: the E2 accepts the ublp from the E1 enzyme and then the E2 often interacts with an E3 enzyme to promote ublp transfer to the target. We report here the crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12). The structure and associated mutational analyses reveal molecular details of Ubc12 recruitment by NEDD8's E1. Interestingly, the E1's Ubc12 binding domain resembles ubiquitin and recruits Ubc12 in a manner mimicking ubiquitin's interactions with ubiquitin binding domains. Structural comparison with E2-E3 complexes indicates that the E1 and E3 binding sites on Ubc12 may overlap and raises the possibility that crosstalk between E1 and E3 interacting with an E2 could influence the specificity and processivity of ublp transfer.

About this Structure

1Y8X is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1., Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA, Mol Cell. 2005 Feb 4;17(3):341-50. PMID:15694336 Page seeded by OCA on Sat May 3 16:01:12 2008

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