1yc5

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[[Image:1yc5.gif|left|200px]]
[[Image:1yc5.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yc5 |SIZE=350|CAPTION= <scene name='initialview01'>1yc5</scene>, resolution 1.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1yc5", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= npdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
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-->
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|DOMAIN=
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{{STRUCTURE_1yc5| PDB=1yc5 | SCENE= }}
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|RELATEDENTRY=[[1yc2|1YC2]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yc5 OCA], [http://www.ebi.ac.uk/pdbsum/1yc5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yc5 RCSB]</span>
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}}
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'''Sir2-p53 peptide-nicotinamide'''
'''Sir2-p53 peptide-nicotinamide'''
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[[Category: Bever, M K.]]
[[Category: Bever, M K.]]
[[Category: Wolberger, C.]]
[[Category: Wolberger, C.]]
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[[Category: nicotinamide]]
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[[Category: Nicotinamide]]
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[[Category: p53]]
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[[Category: P53]]
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[[Category: sir2]]
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[[Category: Sir2]]
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[[Category: sir2tm]]
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[[Category: Sir2tm]]
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[[Category: sirt1]]
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[[Category: Sirt1]]
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[[Category: sirtuin]]
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[[Category: Sirtuin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:08:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:02:25 2008''
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Revision as of 13:08, 3 May 2008

Image:1yc5.gif

Template:STRUCTURE 1yc5

Sir2-p53 peptide-nicotinamide


Overview

Sir2 enzymes form a unique class of NAD(+)-dependent deacetylases required for diverse biological processes, including transcriptional silencing, regulation of apoptosis, fat mobilization, and lifespan regulation. Sir2 activity is regulated by nicotinamide, a noncompetitive inhibitor that promotes a base-exchange reaction at the expense of deacetylation. To elucidate the mechanism of nicotinamide inhibition, we determined ternary complex structures of Sir2 enzymes containing nicotinamide. The structures show that free nicotinamide binds in a conserved pocket that participates in NAD(+) binding and catalysis. Based on our structures, we engineered a mutant that deacetylates peptides by using nicotinic acid adenine dinucleotide (NAAD) as a cosubstrate and is inhibited by nicotinic acid. The characteristics of the altered specificity enzyme establish that Sir2 enzymes contain a single site that participates in catalysis and nicotinamide regulation and provides additional insights into the Sir2 catalytic mechanism.

About this Structure

1YC5 is a Protein complex structure of sequences from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Mechanism of sirtuin inhibition by nicotinamide: altering the NAD(+) cosubstrate specificity of a Sir2 enzyme., Avalos JL, Bever KM, Wolberger C, Mol Cell. 2005 Mar 18;17(6):855-68. PMID:15780941 Page seeded by OCA on Sat May 3 16:08:20 2008

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